Baker D C, Simpson M, Gaunt S D, Corstvet R E
Department of Veterinary Pathology, School of Veterinary Medicine, Louisiana State University, Baton Rouge.
Vet Pathol. 1987 Sep;24(5):449-53. doi: 10.1177/030098588702400513.
Ten dogs were inoculated with Ehrlichia platys (E. platys) from an acutely infected dog. Two dogs were necropsied on each of days 7, 14, 21, 28, and 35 post-inoculation, and tissues were collected and either fixed in formalin or frozen for light microscopic examination of lesions or E. platys antigen localization in tissues. Serum antibody titers to E. platys and serum aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase activities were also determined. The significant light microscopic findings were lymph node follicular hyperplasia and crescent-shaped hemorrhages in the splenic periarteriolar lymphoid sheaths beginning day 7 post-inoculation. There was significant megakaryocyte hyperplasia of bone marrow on days 28 and 35 post-inoculation. Ehrlichia platys antigen was in macrophages at 14 days post-inoculation which corresponded to the initial decline in platelet numbers. Initial thrombocytopenia and splenic crescent-shaped hemorrhages were temporally related, however the degree of lesion development and prominence were not related to subsequent platelet numbers.
从一只急性感染犬身上采集埃氏血小板立克次体（E. platys），接种于10只犬。在接种后的第7、14、21、28和35天，分别对2只犬进行尸检，收集组织，部分固定于福尔马林中，部分冷冻，用于病变的光学显微镜检查或组织中E. platys抗原定位。同时还测定了犬血清中抗E. platys抗体滴度以及血清天冬氨酸转氨酶、丙氨酸转氨酶和碱性磷酸酶活性。光学显微镜下的显著发现包括：接种后第7天开始出现淋巴结滤泡增生以及脾动脉周围淋巴鞘的新月形出血。接种后第28天和第35天，骨髓出现显著的巨核细胞增生。接种后第14天，E. platys抗原存在于巨噬细胞中，这与血小板数量的最初下降相对应。最初的血小板减少与脾新月形出血在时间上相关，但病变发展程度和突出程度与随后的血小板数量无关。
