成年人超重和肥胖与加速度计测量的身体活动与粪便微生物组的关联。

Associations between Accelerometer-Measured Physical Activity and Fecal Microbiota in Adults with Overweight and Obesity.

机构信息

Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL.

出版信息

Med Sci Sports Exerc. 2023 Apr 1;55(4):680-689. doi: 10.1249/MSS.0000000000003096. Epub 2022 Dec 22.

DOI:10.1249/MSS.0000000000003096

PMID:36728974

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9997628/

Abstract

PURPOSE

We aimed to assess whether total daily physical activity (PA), PA intensities, sedentary time (ST), and prolonged ST are associated with differences in the gut microbiota composition or short-chain fatty acid (SCFA) profile of adults with overweight or obesity.

METHODS

Cross-sectional associations between total daily PA (counts per minute), PA intensities (light and moderate-to-vigorous (MVPA)), ST, prolonged ST, and fecal microbiota composition were assessed in adults ( n = 124) between 25 and 45 yr of age with body mass index ≥25 kg·m -2 . Fecal microbiota composition was assessed with 16S rRNA gene sequencing. Daily PA and ST were measured with a hip-worn ActiGraph wGT3X-BT accelerometer.

RESULTS

Daily PA volume and intensity were positively associated with relative abundance of Faecalibacterium ( P = 0.04) and negatively associated with the abundances of Alistipes , Parabacteroides , and Gemmiger ( P = 0.003-0.04) as well as the concentrations of acetate, butyrate, and total SCFA (all P = 0.04). Conversely, ST was negatively associated with abundance of Faecalibacterium but positively associated with the abundances of taxa, including Ruminococcaceae, Parabacteroides , Alistipes , and Gemmiger . Clustering of participants based on whether they met PA recommendations suggested that SCFA profiles differed between individuals who did and did not meet PA recommendations. K-means clustering based on percent of time spent in MVPA and ST also identified differences in fecal microbiota composition between cluster 1 (lower MVPA, higher ST) and cluster 2 (higher MVPA, lower ST), including a higher abundance of Alistipes in cluster 1.

CONCLUSIONS

The current analysis suggests a beneficial association of daily PA on the fecal microbiota and a negative association of ST, particularly with respect to the associations of these variables with the genera Faecalibacterium , a butyrate-producing taxon.

摘要

目的

本研究旨在评估成年人超重或肥胖与总日常体力活动（PA）、PA 强度、久坐时间（ST）和长时间久坐是否与肠道微生物组成或短链脂肪酸（SCFA）谱存在差异有关。

方法

在年龄在 25 至 45 岁、体重指数≥25kg·m -2 的成年人中（n=124），评估总日常 PA（每分钟计数）、PA 强度（低强度和中高强度）、ST、长时间 ST 和粪便微生物组成之间的横断面关联。使用 16S rRNA 基因测序评估粪便微生物组成。每日 PA 和 ST 使用佩戴在髋部的 ActiGraph wGT3X-BT 加速度计进行测量。

结果

每日 PA 量和强度与 Faecalibacterium 的相对丰度呈正相关（P=0.04），与 Alistipes 、Parabacteroides 和 Gemmiger 的丰度以及乙酸盐、丁酸盐和总 SCFA 的浓度呈负相关（P=0.003-0.04）。相反，ST 与 Faecalibacterium 的丰度呈负相关，但与 Ruminococcaceae、Parabacteroides 、Alistipes 和 Gemmiger 等类群的丰度呈正相关。根据是否达到 PA 推荐量对参与者进行聚类，结果表明，达到和未达到 PA 推荐量的个体之间 SCFA 谱存在差异。基于 MVPA 和 ST 时间百分比的 K-均值聚类也确定了粪便微生物组成在簇 1（较低的 MVPA，较高的 ST）和簇 2（较高的 MVPA，较低的 ST）之间存在差异，包括簇 1 中 Alistipes 的丰度更高。