仅撕除 Descemet 膜技术治疗小鼠角膜内皮损伤。
Descemet Stripping Only Technique for Corneal Endothelial Damage in Mice.
机构信息
Laboratory of Corneal Immunology, Transplantation and Regeneration, Department of Ophthalmology, Schepens Eye Research Institute, Massachusetts Eye and Ear, Harvard Medical School, Boston, MA.
出版信息
Cornea. 2023 Apr 1;42(4):470-475. doi: 10.1097/ICO.0000000000003223. Epub 2022 Dec 22.
PURPOSE
Descemet stripping only is an emerging surgical technique used to remove central Descemet membrane and corneal endothelial cells in patients with corneal endothelial disease. Here, we describe a murine model of this procedure to help facilitate basic science investigation and evaluation of postoperative outcomes using this surgical technique.
METHODS
Slitlamp biomicroscopy, central corneal thickness assessment (by optical coherence tomography), and immunohistochemistry were used to assess the model through 7 weeks of follow-up.
RESULTS
Complete removal of the endothelium and Descemet membrane was confirmed by slitlamp biomicroscopy and by histology. Central corneal thickness peaked at day 1 postinjury and then declined over the course of 2 weeks to a stable level of persistent edema. Seven weeks postinjury, immunohistochemical staining for ZO-1 showed the area of Descemet stripping was fully covered by enlarged and dysmorphic corneal endothelial cell. No significant ocular complications were appreciated through the end of the follow-up.
CONCLUSIONS
We demonstrate the feasibility of and provide detailed instructions for a murine model of Descemet stripping only. This model provides a potential in vivo platform to investigate the mechanisms and biology of this emerging surgical procedure.
目的
仅撕除后弹力层是一种新兴的手术技术，用于去除角膜内皮疾病患者的中央后弹力膜和角膜内皮细胞。在这里，我们描述了一种这种手术的小鼠模型，以帮助促进基础科学研究和评估使用这种手术技术的术后结果。
方法
通过 7 周的随访，使用裂隙灯生物显微镜、中央角膜厚度评估（光学相干断层扫描）和免疫组织化学来评估该模型。
结果
通过裂隙灯生物显微镜和组织学证实了内皮细胞和后弹力层的完全去除。中央角膜厚度在损伤后第 1 天达到峰值，然后在 2 周内下降到稳定的持续水肿水平。损伤后 7 周，ZO-1 的免疫组织化学染色显示，后弹力膜撕除区完全被增大和畸形的角膜内皮细胞覆盖。在随访结束时，没有观察到明显的眼部并发症。
结论
我们证明了仅撕除后弹力层的小鼠模型的可行性，并提供了详细的操作说明。该模型为研究这种新兴手术的机制和生物学提供了一个潜在的体内平台。
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