Vlakhov K, Petrov M, Kacheva D, Karaivanov Kh, Sapundzhiev E
Vet Med Nauki. 1987;24(6):61-4.
The results are given of transplantation work with cattle embryos from Czechoslovakia. The embryos were taken in the stage of morula or blastocyst, frozen and stored for one to ten months. Following thawing they were transplanted via surgical methods to local, estrus-synchronized recipient heifers at the Ruzhevo Konare village, Plovdiv district. A calf was born to each of six of the recipients, while two heifers that were inseminated seven days prior to transplantation yielded twins. It was considered to be the result of both the embryo-transplantation and the fertilization of own ova. Discussed are the opportunities and advantages of long-term storage and shipping of animals in the form of frozen embryos.
给出了用捷克斯洛伐克牛胚胎进行移植工作的结果。胚胎取自桑椹胚或囊胚阶段，冷冻并储存一至十个月。解冻后，通过手术方法将它们移植到普罗夫迪夫区鲁热沃科纳雷村当地发情同期化的受体小母牛体内。六头受体母牛各产下一头小牛，而在移植前七天进行人工授精的两头小母牛产下了双胞胎。这被认为是胚胎移植和自身卵子受精两者的结果。文中讨论了以冷冻胚胎形式长期储存和运输动物的机会和优势。
