Richter Kathrin, Wohlrab Johannes
Universitätsklinik und Poliklinik für Dermatologie und Venerologie, Martin-Luther-Universität Halle-Wittenberg, Ernst-Grube-Straße 40, 06114, Halle (Saale), Deutschland.
An-Institut für angewandte Dermatopharmazie, Martin-Luther-Universität Halle-Wittenberg, Halle (Saale), Deutschland.
Dermatologie (Heidelb). 2023 Mar;74(3):171-181. doi: 10.1007/s00105-023-05112-x. Epub 2023 Feb 2.
Preservatives are used to stabilize topical preparations and protect the user from the influence of pathogenic microbes. After the application of a topical preparation, the matrix undergoes a metamorphosis, and by proportional evaporation of the hydrophilic phase the preservative may accumulate on the skin surface. This is believed to lead to antiseptic effects and may influence the diversity of the cutaneous microbiota. The regulation of the cutaneous microbiome and the associated influencing factors is a complex system that results in highly individualized conditions. Therefore, investigations on the influence of defined interventions are methodologically difficult. In the present proof-of-concept study, potential antiseptic effects of preservatives were investigated in a combination of in vitro and in vivo methods using microbiological culture tests. In addition, the investigations served to develop a clinical study design to answer further questions and use of an extended range of methods. The results support the hypothesis of an antiseptic effect of the tested preservatives (methyl-4-hydroxybenzoate and propyl-4-hydroxybenzoate, potassium sorbate and propylene glycol) on prominent reference bacteria, which could also be observed in clinical settings.
防腐剂用于稳定局部用制剂，并保护使用者免受致病微生物的影响。局部用制剂应用后，基质会发生变化，通过亲水相的比例蒸发，防腐剂可能会积聚在皮肤表面。据信这会导致抗菌作用，并可能影响皮肤微生物群的多样性。皮肤微生物组的调节及相关影响因素是一个复杂的系统，会导致高度个体化的情况。因此，关于特定干预措施影响的研究在方法上具有难度。在本概念验证研究中，使用微生物培养试验，通过体外和体内方法相结合，研究了防腐剂的潜在抗菌作用。此外，这些研究旨在制定一项临床研究设计，以回答更多问题并使用更广泛的方法。结果支持了受试防腐剂（对羟基苯甲酸甲酯和对羟基苯甲酸丙酯、山梨酸钾和丙二醇）对重要参考细菌具有抗菌作用的假设，这在临床环境中也可观察到。
