School of Public Health, Health Science Center, Shenzhen University, Shenzhen, Guangdong 518060, China.
Department of Food Science and Engineering, College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, Guangdong 518060, China.
Int J Biol Macromol. 2020 Jun 1;152:189-198. doi: 10.1016/j.ijbiomac.2020.02.248. Epub 2020 Feb 24.
In this study, pea protein isolate (PPI) nanoparticles were fabricated with calcium-induced cross-linking and the potential as a nano-carrier for protecting resveratrol (RES) from degradation as well as improving its antioxidant activities was investigated. Ca ions concentration and pH value had significant impacts on the formation of PPI nanoparticles. Dissociation assays suggested that PPI nanoparticles were mainly formed and stabilized by Ca ions induced salt-bridge, hydrophobic interaction, and hydrogen bonding. Encapsulation efficiency (EE) and Loading amount (LA) of RES in PPI nanoparticles was 74.08%, and 30.24 μg/mg protein, respectively. Fluorescence emission results suggested that the formation of RES-PPI nanoparticles was primarily driven with hydrophobic interaction. AFM results clearly indicated that both RES-PPI nanocomplexes and RES-PPI nanoparticles were nano-scale, spherical shaped and distributed uniformly. RES-PPI nanoparticles exhibited higher physicochemical stability (Z-average diameter stability and RES retention) than RES-SPI nanocomplexes. Antioxidant ability of RES can be remarkably enhanced with both PPI-based nano-delivery systems. Ca ions induced PPI nanoparticles obtained in this study have the great potential as functional delivery systems for hydrophobic nutraceuticals in food, and pharmaceutical industry.
在这项研究中，采用钙离子交联法制备豌豆蛋白分离物（PPI）纳米粒子，并研究其作为纳米载体保护白藜芦醇（RES）免受降解以及提高其抗氧化活性的潜力。钙离子浓度和 pH 值对 PPI 纳米粒子的形成有显著影响。离解实验表明，PPI 纳米粒子主要通过钙离子诱导的盐桥、疏水相互作用和氢键形成和稳定。RES 在 PPI 纳米粒子中的包封效率（EE）和载药量（LA）分别为 74.08%和 30.24μg/mg 蛋白。荧光发射结果表明，RES-PPI 纳米粒子的形成主要是由疏水相互作用驱动的。原子力显微镜（AFM）结果清楚地表明，RES-PPI 纳米复合物和 RES-PPI 纳米粒子均为纳米级、球形且分布均匀。与 RES-SPI 纳米复合物相比，RES-PPI 纳米粒子具有更高的物理化学稳定性（Z 均粒径稳定性和 RES 保留率）。RES 的抗氧化能力可以通过这两种基于 PPI 的纳米递药系统得到显著增强。本研究中获得的钙离子诱导 PPI 纳米粒子有望成为食品和制药工业中疏水性营养保健品的功能性递送系统。
