Two new alkaloids from a strain of endophytic fungus sp.

19-Hydroxybrevianamide M (1) and 6 R-methoxybrevianamide V (2), two new alkaloids, were isolated from an extract of the endophytic fungus sp. JNU18HC0517J, together with six known analogues (). Their structures were elucidated by extensive spectroscopic analyses, NMR calculations, and ECD calculations. 6 -methoxybrevianamide V (2) was the first L-proline indole DKP alkaloid with substitution at C-6 on the proline ring. Furthermore, the cytotoxities and antimicrobial activities of these isolated compounds were also evaluated. Compound exhibited moderate antibacterial activity against 209 P with a minimal inhibitory concentration (MIC) value of 16 µg/ml.[Figure: see text].