Sino-French Hoffmann Institute, School of Basic Medical Science, State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangzhou, China.
Guangdong South China Vaccine Co. Ltd, Guangzhou, China.
Expert Rev Proteomics. 2022 Jul-Dec;19(7-12):311-324. doi: 10.1080/14789450.2023.2176756. Epub 2023 Feb 13.
Chronic obstructive pulmonary disease (COPD) was the third leading cause of global death in 2019, causing a huge economic burden to society. Therefore, it is urgent to identify specific phenotypes of COPD patients through early detection, and to promptly treat exacerbations. The field of phosphoproteomics has been a massive advancement, compelled by the developments in mass spectrometry, enrichment strategies, algorithms, and tools. Modern mass spectrometry-based phosphoproteomics allows understanding of disease pathobiology, biomarker discovery, and predicting new therapeutic modalities.
In this article, we present an overview of phosphoproteomic research and strategies for enrichment and fractionation of phosphopeptides, identification of phosphorylation sites, chromatographic separation and mass spectrometry detection strategies, and the potential application of phosphorylated proteomic analysis in the diagnosis, treatment, and prognosis of COPD disease.
The role of phosphoproteomics in COPD is critical for understanding disease pathobiology, identifying potential biomarkers, and predicting new therapeutic approaches. However, the complexity of COPD requires the more comprehensive understanding that can be achieved through integrated multi-omics studies. Phosphoproteomics, as a part of these multi-omics approaches, can provide valuable insights into the underlying mechanisms of COPD.
慢性阻塞性肺疾病（COPD）是 2019 年全球第三大致死原因，给社会带来了巨大的经济负担。因此，通过早期检测识别 COPD 患者的特定表型，并及时治疗恶化，这一点非常紧迫。由于质谱、富集策略、算法和工具的发展，磷酸化蛋白质组学领域取得了巨大进展。基于现代质谱的磷酸化蛋白质组学可以帮助我们理解疾病的病理生物学、发现生物标志物，并预测新的治疗方式。
在这篇文章中，我们介绍了磷酸化蛋白质组学的研究概况，以及磷酸肽的富集和分级、磷酸化位点的鉴定、色谱分离和质谱检测策略，以及磷酸化蛋白质组分析在 COPD 疾病的诊断、治疗和预后中的潜在应用。
磷酸化蛋白质组学在 COPD 中的作用对于理解疾病的病理生物学、识别潜在的生物标志物和预测新的治疗方法至关重要。然而，COPD 的复杂性需要通过综合的多组学研究来获得更全面的理解。磷酸化蛋白质组学作为这些多组学方法的一部分，可以为 COPD 的潜在机制提供有价值的见解。
