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从原发性实验梅毒病变的新鲜和冷冻针吸物中体外分离苍白密螺旋体亚种苍白密螺旋体。

In Vitro Isolation of Treponema pallidum subsp. pallidum From Fresh and Frozen Needle Aspirates of Primary Experimental Syphilis Lesions.

机构信息

From the Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, WA.

Keck School of Medicine, University of Southern California, Los Angeles, CA.

出版信息

Sex Transm Dis. 2023 Mar 1;50(3):180-183. doi: 10.1097/OLQ.0000000000001739. Epub 2022 Nov 19.

DOI:10.1097/OLQ.0000000000001739
PMID:36730573
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9906972/
Abstract

Isolation of Treponema pallidum subsp. pallidum strains still relies on rabbit intratesticular inoculation of clinical samples. In this article, we report an alternative isolation approach based on the inoculation of fresh and frozen needle aspirates of primary experimental lesions into culture plates suitable for in vitro propagation of the syphilis agent.

摘要

苍白密螺旋体亚种苍白密螺旋体菌株的分离仍然依赖于将临床样本接种到兔睾丸内。在本文中，我们报告了一种替代的分离方法，该方法基于将新鲜和冷冻的原发性实验性损害的针吸物接种到适合梅毒螺旋体体外繁殖的培养板中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5129/9906972/e0ce9224caf0/nihms-1851079-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5129/9906972/029b54ab53c5/nihms-1851079-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5129/9906972/f2606f8b857d/nihms-1851079-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5129/9906972/e0ce9224caf0/nihms-1851079-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5129/9906972/029b54ab53c5/nihms-1851079-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5129/9906972/f2606f8b857d/nihms-1851079-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5129/9906972/e0ce9224caf0/nihms-1851079-f0003.jpg

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Sex Transm Dis. 2024 Dec 1;51(12):775-779. doi: 10.1097/OLQ.0000000000002057. Epub 2024 Jul 24.