从原发性实验梅毒病变的新鲜和冷冻针吸物中体外分离苍白密螺旋体亚种苍白密螺旋体。
In Vitro Isolation of Treponema pallidum subsp. pallidum From Fresh and Frozen Needle Aspirates of Primary Experimental Syphilis Lesions.
机构信息
From the Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, WA.
Keck School of Medicine, University of Southern California, Los Angeles, CA.
出版信息
Sex Transm Dis. 2023 Mar 1;50(3):180-183. doi: 10.1097/OLQ.0000000000001739. Epub 2022 Nov 19.
Abstract
Isolation of Treponema pallidum subsp. pallidum strains still relies on rabbit intratesticular inoculation of clinical samples. In this article, we report an alternative isolation approach based on the inoculation of fresh and frozen needle aspirates of primary experimental lesions into culture plates suitable for in vitro propagation of the syphilis agent.
摘要
苍白密螺旋体亚种苍白密螺旋体菌株的分离仍然依赖于将临床样本接种到兔睾丸内。在本文中，我们报告了一种替代的分离方法，该方法基于将新鲜和冷冻的原发性实验性损害的针吸物接种到适合梅毒螺旋体体外繁殖的培养板中。
相似文献
1
In Vitro Isolation of Treponema pallidum subsp. pallidum From Fresh and Frozen Needle Aspirates of Primary Experimental Syphilis Lesions.从原发性实验梅毒病变的新鲜和冷冻针吸物中体外分离苍白密螺旋体亚种苍白密螺旋体。
Sex Transm Dis. 2023 Mar 1;50(3):180-183. doi: 10.1097/OLQ.0000000000001739. Epub 2022 Nov 19.
2
Treponema pallidum genetic diversity and its implications for targeted vaccine development: A cross-sectional study of early syphilis cases in Southwestern Colombia.苍白密螺旋体遗传多样性及其对靶向疫苗开发的影响：哥伦比亚西南部早期梅毒病例的横断面研究。
PLoS One. 2024 Jul 19;19(7):e0307600. doi: 10.1371/journal.pone.0307600. eCollection 2024.
3
In vitro isolation of contemporary Treponema pallidum strains directly from clinical samples of syphilis patients.直接从梅毒患者临床样本中体外分离当代梅毒螺旋体菌株。
Clin Microbiol Infect. 2025 Jun;31(6):1061-1065. doi: 10.1016/j.cmi.2025.03.017. Epub 2025 Mar 25.
4
HIV Infection Modifies the Role of Prior Treponema pallidum Infection in the Clinical Presentation of Early Syphilis Among Adult Patients From Sexually Transmitted Infection Clinics in Peru.HIV 感染改变了梅毒螺旋体既往感染在秘鲁性传播感染门诊成年早期梅毒患者临床表型中的作用。
Sex Transm Dis. 2024 Jun 1;51(6):415-419. doi: 10.1097/OLQ.0000000000001950. Epub 2024 Feb 19.
5
Molecular typing of Treponema pallidum: a systematic review and meta-analysis.梅毒螺旋体的分子分型：系统评价和荟萃分析。
PLoS Negl Trop Dis. 2011 Nov;5(11):e1273. doi: 10.1371/journal.pntd.0001273. Epub 2011 Nov 8.
6
High rates of Treponema pallidum, Neisseria gonorrhoeae, Chlamydia trachomatis, or Trichomonas vaginalis co-infection in people with HIV: a systematic review and meta-analysis.HIV感染者中梅毒螺旋体、淋病奈瑟菌、沙眼衣原体或阴道毛滴虫的高合并感染率：一项系统评价和荟萃分析。
Eur J Clin Microbiol Infect Dis. 2025 Jan;44(1):1-15. doi: 10.1007/s10096-024-04966-w. Epub 2024 Oct 28.
7
Treponema pallidum infection in asymptomatic persons: A puzzling scenario in the Canary Islands (Spain) (2001-2020).无症状人群中的梅毒螺旋体感染：西班牙加那利群岛（2001 - 2020年）的一个令人困惑的情况
PLoS One. 2025 Jul 8;20(7):e0325073. doi: 10.1371/journal.pone.0325073. eCollection 2025.
8
Comparison of Diagnostic Accuracy of PCR Targeting the 47-Kilodalton Protein Membrane Gene of Treponema pallidum and PCR Targeting the DNA Polymerase I Gene: Systematic Review and Meta-analysis.针对梅毒螺旋体47千道尔顿蛋白膜基因的聚合酶链反应（PCR）与针对DNA聚合酶I基因的PCR诊断准确性比较：系统评价与荟萃分析
J Clin Microbiol. 2015 Nov;53(11):3522-9. doi: 10.1128/JCM.01619-15. Epub 2015 Aug 26.
9
High quality transcriptome profiling confirms the transcriptional landscape of Treponema pallidum subsp. pallidum.高质量转录组分析确定了梅毒螺旋体苍白亚种的转录图谱。
Sci Rep. 2025 Jul 2;15(1):23272. doi: 10.1038/s41598-025-06583-9.
10
A Novel Treponema pallidum Subspecies pallidum Strain Associated With a Painful Oral Lesion Is a Member of a Potentially Emerging Nichols-Related Subgroup.一种与疼痛性口腔损害相关的新型苍白密螺旋体亚种苍白密螺旋体菌株是一种潜在新兴的尼科尔斯相关亚群的成员。
Sex Transm Dis. 2024 Jul 1;51(7):486-492. doi: 10.1097/OLQ.0000000000001971. Epub 2024 Jun 3.
引用本文的文献
1
Expanding Horizons in Syphilis Treatment: Challenges, Advances, and Opportunities for Alternative Antibiotics.梅毒治疗的拓展视野：挑战、进展及替代抗生素的机遇
Curr HIV/AIDS Rep. 2025 Mar 13;22(1):22. doi: 10.1007/s11904-025-00725-4.
2
Congenital Syphilis-Comprehensive Narrative Review of Alternative Antibiotic Treatment for Use in Neonates.先天性梅毒——替代抗生素治疗新生儿的全面叙述性综述。
Sex Transm Dis. 2024 Dec 1;51(12):775-779. doi: 10.1097/OLQ.0000000000002057. Epub 2024 Jul 24.
Zotero 插件