Putra Ivan Permana, Aimi Tadanori, Shimomura Norihiro
The United Graduate School of Agricultural Sciences, Tottori University, 4-101 Koyamacho Minami, Tottori 680-8553, Japan.
Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680, Indonesia.
Mycologia. 2023 Mar-Apr;115(2):216-224. doi: 10.1080/00275514.2022.2162799. Epub 2023 Feb 2.
While observing mycelial features of the ectomycorrhizal fungus , we noted the formation of hyphal coils in laboratory cultures. The coiled hyphae initially formed at the hyphal tip in the presence or absence of the plant host (), on both rich and poor modified Melin-Norkrans media. Hyphal coils formed from the hyphal tips toward the middle of the hyphae, with rope-like hyphal strands fused as extensive circular coils. Hyphal coils were generally oval and circular. Coils were composed of 1-5 layers of hyphae. Presence of the host had a significant impact on the diameter of the coil loops, which was consistently wider without the host than with the host present on both media. In addition, the terminal part of the mature coils was melanized and separated from the unmelanized coil by a septum. The melanized coils resembled thick condensed hyphae and were detached and scattered throughout the fungal colony. The observation of morphological characteristics suggested that mature coils detached into the mycelia were thromboplerous hyphae. This is the first report on hyphal coil morphogenesis and its potential relationship to the initial development of thromboplerous hyphae.
在观察外生菌根真菌的菌丝体特征时，我们在实验室培养物中注意到了菌丝盘绕的形成。在有或没有植物宿主的情况下，在富营养和贫营养改良的梅林 - 诺克兰斯培养基上，盘绕菌丝最初都在菌丝顶端形成。菌丝盘绕从菌丝顶端向菌丝中部形成，绳状菌丝束融合成广泛的圆形盘绕。菌丝盘绕通常为椭圆形和圆形。盘绕由1 - 5层菌丝组成。宿主的存在对盘绕环的直径有显著影响，在两种培养基上，没有宿主时盘绕环的直径始终比有宿主时更宽。此外，成熟盘绕的末端部分黑色素化，并通过隔膜与未黑色素化的盘绕分开。黑色素化的盘绕类似于粗缩的菌丝，会脱离并散布在整个真菌菌落中。形态特征观察表明，脱落在菌丝体中的成熟盘绕是血栓形成性菌丝。这是关于菌丝盘绕形态发生及其与血栓形成性菌丝初始发育潜在关系的首次报道。
