The United Graduate School of Agricultural Sciences, Tottori University, 4-101 Koyama-cho Minami, Tottori 680-8553, Japan.
Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680, Indonesia.
Mycologia. 2022 Jul-Aug;114(4):670-681. doi: 10.1080/00275514.2022.2071119. Epub 2022 Jun 9.
The impact of host plant on the mycelial features of mycorrhizal symbiont and its characteristics has been poorly investigated. This study aimed to compare and quantify (statistically tested) some of the mycelial features of an ectomycorrhiza (ECM)-forming fungus with and without the ECM host. The ECM-forming fungus, , inoculated with or without on both rich and poor nutrient media, was observed under laboratory conditions. On rich medium, fungi with the host had the highest colony diameter and consistently produced the highest hyphal length relative to fungi on other media. Thus, the host had a significant impact on the mycelium production of in both rich and poor media. Further, fungi without the host had a higher number of hyphal anastomoses per hyphal length on both poor and rich media than fungi with the host in the same medium. Anastomosis, which refers to the fusion of two parallel hyphae, was evident in all experiments. However, there was no significant impact of the host on the number of hyphal anastomoses. In addition, fungi without the host had more frequent hyphal branching on both rich and poor media than fungi with the host. The occurrence of a host only had a significant impact on the formation of the hyphal branch on poor medium. Further, a chlamydospore-like structure was identified, which had a higher diameter when formed with the host on both rich and poor media. The present data provide new insights into the host plant's impact on the mycelial features of ECM-forming fungi.
宿主植物对共生菌菌丝特征及其特性的影响尚未得到充分研究。本研究旨在比较和量化（经统计学检验）形成外生菌根（ECM）真菌在有无 ECM 宿主时的一些菌丝特征。在实验室条件下，观察接种了或未接种 ECM 宿主的 ECM 形成真菌在丰富和贫瘠营养培养基上的生长情况。在丰富的培养基上，与其他培养基相比，有宿主的真菌具有最大的菌落直径，且始终产生最长的菌丝长度。因此，宿主对富营养和贫营养培养基中真菌的菌丝生长有显著影响。此外，在贫营养和富营养培养基上，没有宿主的真菌的菌丝长度上的菌丝吻合数量高于有宿主的真菌。吻合是指两条平行菌丝的融合，在所有实验中都很明显。然而，宿主对菌丝吻合数量没有显著影响。此外，没有宿主的真菌在富营养和贫营养培养基上的分支频率都高于有宿主的真菌。只有宿主的存在对贫营养培养基上的菌丝分支形成有显著影响。此外，还鉴定了一种类似于厚垣孢子的结构，当它在富营养和贫营养培养基上与宿主一起形成时，其直径更大。本研究数据为宿主植物对形成 ECM 真菌的菌丝特征的影响提供了新的见解。
