Int J Gynecol Pathol. 2023 May 1;42(3):293-300. doi: 10.1097/PGP.0000000000000884. Epub 2022 Oct 10.
In this study, we compared the placental T-cadherin staining intensity of pregnant women with placenta percreta (PP) and asymmetrical fetal growth restriction (FGR) compared with healthy control pregnancies. Placental T-cadherin levels of the placenta of 86 pregnant women in total, 25 with FGR, 30 with healthy pregnant subjects, and 31 with PP, were examined using monoclonal anti-T-cadherin (CDH13) antibody for immunohistochemical examination. In immunohistochemistry, H -scores were used for each group to compare the expression of T-cadherin in extravillous trophoblast (EVT) cells. T-cadherin H -score of EVTs was highest in the FGR group and the lowest in the PP group. The difference in H -score between the FGR group and the control group was not statistically significant ( P =0.344). The difference between the PP group and the other 2 groups was significant ( P <0.0001). Multivariable linear regression analysis with a stepwise elimination method was performed in order to identify demographic and clinical parameters with significant effects on the T-cadherin H -score of EVTs. The estimation results identified only the disease group as a significant predictor of the H -score of EVTs ( R2 =0.340, P <0.0001). The highest T-cadherin H -score of EVTs was found in the FGR group and the lowest in the PP group. The low T-cadherin H-score values in the PP group suggest that low T-cadherin EVTs may be associated with increased placental invasion. Likewise, despite the statistical insignificance, a higher T-cadherin H -score of EVTs in FGR compared with controls implies a decreased invasiveness of the placenta in FGR.
在这项研究中，我们比较了胎盘侵袭性疾病胎盘植入（PP）和非对称性胎儿生长受限（FGR）孕妇与正常妊娠孕妇胎盘 T-钙黏蛋白染色强度。使用抗 T-钙黏蛋白（CDH13）单克隆抗体进行免疫组织化学检查，共检查了 86 名孕妇的胎盘 T-钙黏蛋白水平，其中 25 名患有 FGR，30 名正常孕妇，31 名患有 PP。在免疫组化中，使用 H 评分比较各组绒毛外滋养细胞（EVT）中 T-钙黏蛋白的表达。EVT 中 T-钙黏蛋白 H 评分在 FGR 组最高，在 PP 组最低。FGR 组与对照组 H 评分差异无统计学意义（P=0.344）。PP 组与其他两组的差异有统计学意义（P<0.0001）。采用逐步消除法进行多变量线性回归分析，以确定对 EVT 中 T-钙黏蛋白 H 评分有显著影响的人口统计学和临床参数。估计结果仅确定疾病组是 EVT H 评分的显著预测因子（R2=0.340，P<0.0001）。EVT 中 T-钙黏蛋白 H 评分最高的是 FGR 组，最低的是 PP 组。PP 组 T-钙黏蛋白 H 评分较低提示低 T-钙黏蛋白 EVT 可能与胎盘侵袭性增加有关。同样，尽管统计学上无显著性意义，但与对照组相比，FGR 中 EVT 的 T-钙黏蛋白 H 评分较高意味着 FGR 中胎盘的侵袭性降低。
