Suarez Emaly M, Kelson Victoria C, Kiser Jennifer N, Davenport Kimberly M, Murdoch Brenda M, Neibergs Holly L
Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA.
Washington Animal Disease Diagnostic Laboratory, Washington State University, Pullman, WA 99164, USA.
Genes (Basel). 2024 Nov 22;15(12):1498. doi: 10.3390/genes15121498.
The dairy industry relies on reproductive efficiency to maintain efficient milk production. Spontaneous abortion (SA), defined as pregnancy loss between gestation days 42 and 260, occurred in 4.5% of the artificially inseminated (AI) Holstein heifers and 31.6% of the embryo transfer (ET) recipient Holstein heifers that received in vitro-produced frozen embryos on a single dairy farm in Idaho. A genome-wide association analysis (GWAA) was performed to identify the associations (FDR < 0.05) with SA in heifers that were bred by AI (1351 controls that delivered at term and 63 cases that aborted) that conceived following the first insemination, as well as in 59 controls and 273 cases of ET recipient heifers pregnant from the first ET. There were 216 loci and 413 positional candidate genes associated (FDR < 0.05) with SA in the heifers bred by AI in a recessive model and no loci associated with SA in the ET recipients. The identification of loci associated with SA in the heifers bred by AI may be used to reduce fetal loss through genomic selection.
乳制品行业依赖繁殖效率来维持高效的牛奶生产。自然流产（SA）定义为妊娠第42天至260天之间的妊娠丢失，在爱达荷州一个奶牛场接受体外生产的冷冻胚胎的人工授精（AI）荷斯坦青年母牛中，自然流产发生率为4.5%，在胚胎移植（ET）受体荷斯坦青年母牛中为31.6%。进行了全基因组关联分析（GWAA），以确定首次人工授精后受孕的人工授精青年母牛（1351头足月分娩的对照和63例流产病例）以及首次胚胎移植后怀孕的59例对照和273例胚胎移植受体青年母牛中与自然流产相关的关联（错误发现率<0.05）。在隐性模型中，有216个位点和413个位置候选基因与人工授精青年母牛的自然流产相关（错误发现率<0.05），而在胚胎移植受体中没有与自然流产相关的位点。确定与人工授精青年母牛自然流产相关的位点可用于通过基因组选择减少胎儿丢失。
