耐药伤寒沙门氏菌的分子鉴定与差异蛋白质组学

Molecular identification and differential proteomics of drug resistant Salmonella Typhi.

作者信息

Safi Aziz Ur Rehman, Bendixen Emoke, Rahman Hazir, Khattak Baharullah, Wu Wei, Ullah Waheed, Khan Nasar, Ali Farhad, Yasin Nusrat, Qasim Muhammad

机构信息

Department of Microbiology, Kohat University of Science and Technology, Kohat Pakistan.

Department of Molecular Biology and Genetics, Aarhus University, Aarhus C Denmark.

出版信息

Diagn Microbiol Infect Dis. 2023 Apr;105(4):115883. doi: 10.1016/j.diagmicrobio.2022.115883. Epub 2022 Dec 21.

DOI:10.1016/j.diagmicrobio.2022.115883

PMID:36731197

Abstract

This study aimed to elucidate differentially expressed proteins in drug resistant Salmonella Typhi. Among 100 samples, S. typhi were identified in 43 samples. In drug susceptibility profile, 95.3% (41/43), 80% (35/43) and 70% (30/43) resistances were observed against Nalidixic acid, Ampicillin, and Chloramphenicol respectively. No resistance was observed against Imipenum and Azithromycin while only 11% (5/43) isolates were found resistant to Ceftriaxone. Mass spectrometric differential analysis resulted in 23 up-regulated proteins in drug resistant isolates. Proteins found up-regulated are involved in virulence (vipB, galU, tufA, and lpp1), translation (rpsF, rpsG, rplJ, and rplR), antibiotic resistance (zwf, phoP, and ompX), cell metabolism (metK, ftsZ, pepD, and secB), stress response (ridA, rbfA, and dps), housekeeping (gapA and eno) and hypothetical proteins including ydfZ, t1802, and yajQ. These proteins are of diverse nature and functions but highly interconnected. Further characterization may be helpful for elucidation of new biomarker proteins and therapeutic drug targets.

摘要

本研究旨在阐明耐药伤寒沙门氏菌中差异表达的蛋白质。在100个样本中，43个样本鉴定出伤寒沙门氏菌。在药敏试验中，分别观察到对萘啶酸、氨苄西林和氯霉素的耐药率为95.3%（41/43）、80%（35/43）和70%（30/43）。对亚胺培南和阿奇霉素未观察到耐药性，而仅11%（5/43）的分离株对头孢曲松耐药。质谱差异分析导致耐药分离株中有23种上调蛋白。上调的蛋白涉及毒力（vipB、galU、tufA和lpp1）、翻译（rpsF、rpsG、rplJ和rplR）、抗生素耐药性（zwf、phoP和ompX）、细胞代谢（metK、ftsZ、pepD和secB）、应激反应（ridA、rbfA和dps）、管家（gapA和eno）以及包括ydfZ、t1802和yajQ在内的假设蛋白。这些蛋白质具有不同的性质和功能，但高度相互关联。进一步的表征可能有助于阐明新的生物标志物蛋白和治疗药物靶点。