College of Plant Protection, State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing Agricultural University, Nanjing 210095, P. R. China.
Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, P. R. China.
J Agric Food Chem. 2023 Feb 15;71(6):2914-2923. doi: 10.1021/acs.jafc.2c06928. Epub 2023 Feb 2.
A type II polyketide synthase biosynthetic gene cluster () was identified in CGMCC 4.1086 via genome mining. The heterologous expression of the cryptic gene cluster in J1074 generated dimerized aromatic polyketide naphthocyclinones (), whose structures were determined via extensive analysis using nuclear magnetic resonance and high-resolution electrospray ionization mass spectroscopy. The biological pathway of naphthocyclinone synthesis was revealed via in vivo gene deletion, in vitro biochemical reactions, and comparative genomics. Remarkably, played a crucial role in inhibiting and , with EC values of 6.1 and 20.2 μg/mL, respectively. Furthermore, exhibited a potent protective effect against and in greenhouse tests.
通过基因组挖掘，在 CGMCC 4.1086 中鉴定出一个 II 型聚酮合酶生物合成基因簇 ()。在 J1074 中异源表达该隐性基因簇，生成二聚化芳香聚酮萘环酮（），通过核磁共振和高分辨电喷雾电离质谱的广泛分析确定了其结构。通过体内基因缺失、体外生化反应和比较基因组学揭示了萘环酮合成的生物途径。值得注意的是，对抑制和具有关键作用，EC 值分别为 6.1 和 20.2μg/mL。此外，在温室试验中，对和具有很强的保护作用。
