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[真空下石蜡包埋的简易方法]

[Simple method of embedding in paraffin under vacuum].

作者信息

Krivonosov L K

出版信息

Arkh Patol. 1978;40(11):75-6.

PMID:367332
Abstract

A method of embedding into paraffin under vacuum is proposed. Pieces of tissues are placed into a glass jar containing melted paraffin from which the air is pumped out. The jar is permanently placed into TC-80 thermostate. The air-removing hose is placed inside the thermostate through the opening in its upper wall meant for the control thermometer. The air is pumped out by any vacuum pump with electric drive. The proposed method is simple, provides for a good saturation with paraffin, shortens the time of tissue contact with melted paraffin, and gives thin sections of good quality.

摘要

提出了一种真空下石蜡包埋的方法。将组织块放入装有熔化石蜡的玻璃罐中,然后抽出空气。该玻璃罐永久放置在TC - 80恒温器中。排气软管通过恒温器上壁用于控制温度计的开口放置在恒温器内。用任何电动真空泵抽出空气。所提出的方法简单,能使石蜡充分浸透,缩短组织与熔化石蜡接触的时间,并能得到高质量的薄片。

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