Kovalev S P
Tsitologiia. 1979 Nov;21(11):1372-3.
A method is proposed of cell cultivation on the microscopic glass. Glass rings with inner diameter of 6 mm are fixed on prepared glasses. A simple of 0.2 ml cell suspension is poured into so-formed microcups. The cultivation is carried out in hermetic Petri dishes under conditions of the 5% CO2 atmospheric saturation at 37 degrees C.
提出了一种在显微镜载玻片上进行细胞培养的方法。内径为6毫米的玻璃环固定在制备好的载玻片上。将0.2毫升细胞悬液倒入这样形成的微杯中。培养在密封的培养皿中于37摄氏度、5%二氧化碳饱和气氛条件下进行。
