基于可变形镜的光声遥感（PARS）显微镜用于深度扫描。
Deformable mirror-based photoacoustic remote sensing (PARS) microscopy for depth scanning.
作者信息
Mukhangaliyeva Lyazzat, Kocer Samed, Warren Alkris, Bell Kevan, Boktor Marian, Yavuz Mustafa, Abdel-Rahman Eihab, Haji Reza Parsin
机构信息
PhotoMedicine Labs, Department of Systems Design Engineering, University of Waterloo, 200 University Ave W, Waterloo, ON, N2L 3G1, Canada.
Department of Mechanical and Mechatronics Engineering, University of Waterloo, 200 University Ave W, Waterloo, ON, N2L 3G1, Canada.
出版信息
Biomed Opt Express. 2022 Oct 7;13(11):5643-5653. doi: 10.1364/BOE.471770. eCollection 2022 Nov 1.
Optically shifting the focal plane to allow depth scanning of delicate biological structures and processes in their natural environment offers an appealing alternative to conventional mechanical scanning. Our technique uses a deformable mirror-based photoacoustic remote sensing microscopy (PARS) with a focus shifting of Δz ∼ 240 µm. We achieve this by integrating a deformable mirror that functions as a varifocal mirror for axial scanning. First, the system's focal shift capability was demonstrated with USAF resolution targets and carbon fiber phantoms, followed by visualizations of blood vessels in chicken embryo chorioallantoic membrane (CAM). This work represents an initial step toward developing a non-contact, label-free, and aberration-free PARS imaging system with axial scanning capability.
通过光学方式移动焦平面以允许在自然环境中对脆弱的生物结构和过程进行深度扫描，这为传统机械扫描提供了一种有吸引力的替代方法。我们的技术使用基于可变形镜的光声遥感显微镜（PARS），其焦移量约为Δz ∼ 240 µm。我们通过集成一个可变形镜来实现这一点，该可变形镜用作轴向扫描的变焦镜。首先，使用美国空军分辨率靶标和碳纤维模型展示了系统的焦移能力，随后对鸡胚绒毛尿囊膜（CAM）中的血管进行了可视化。这项工作代表了朝着开发具有轴向扫描能力的非接触、无标记和无像差的PARS成像系统迈出的第一步。
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