Liang Siqi, Zhou Jiasheng, Yang Wenzhao, Chen Sung-Liang
Opt Lett. 2022 Jan 1;47(1):18-21. doi: 10.1364/OL.446787.
Photoacoustic microscopy (PAM) is a unique tool for biomedical applications because it can visualize optical absorption contrast in vivo. Recently, non-contact PAM based on non-interferometric photoacoustic remote sensing (PARS), termed PARS microscopy, has shown promise for selected imaging applications. A variety of superluminescent diodes (SLDs) have been employed in the PARS microscopy system as the interrogation light source. Here, we investigate the use of a low-cost laser diode (LD) as the interrogation light source in PARS microscopy, termed PARS-LD. A side-by-side comparison of PARS-LD and a PARS microscopy system using an SLD was conducted that showed comparable performance in terms of resolution and signal-to-noise ratio. More importantly, for the first time to our knowledge, in vivo PAM imaging of mouse brain vessels was conducted in a non-contact manner, and the results show that PARS-LD provides great performance.
光声显微镜（PAM）是生物医学应用中的一种独特工具，因为它能够在体内可视化光吸收对比度。最近，基于非干涉光声遥感（PARS）的非接触式PAM，即PARS显微镜，在某些成像应用中显示出了前景。多种超发光二极管（SLD）已被用于PARS显微镜系统作为询问光源。在此，我们研究在PARS显微镜中使用低成本激光二极管（LD）作为询问光源，即PARS-LD。对PARS-LD和使用SLD的PARS显微镜系统进行了并排比较，结果表明在分辨率和信噪比方面具有可比的性能。更重要的是，据我们所知，首次以非接触方式对小鼠脑血管进行了体内PAM成像，结果表明PARS-LD具有出色的性能。
