Wang Xin-Liang, Peng Ling, Wang Jian, Liu Jing-Lei, Jia Jing-Jing, Tang Li-Ping
Editorial Department of Journal of Binzhou University, Binzhou University, 391 Huanghe 5th Road, Binzhou, 256603 Shandong People's Republic of China.
Shandong Key Laboratory of Eco-Environmental Science for the Yellow River Delta, Binzhou University, 391 Huanghe 5th Road, Binzhou, 256603 Shandong People's Republic of China.
Physiol Mol Biol Plants. 2023 Jan;29(1):11-22. doi: 10.1007/s12298-022-01275-4. Epub 2023 Jan 5.
Saline-alkali stress is a universal abiotic stress factor limiting fruit tree cultivation worldwide. Apple (× Borkh.) is one of the fruits with the largest yields worldwide. Tea crabapple ( Rehd. var. Jiang) is a type of common apple rootstock in China. Because facultative apomixis occurs in this species, it is often used in molecular research. The present study investigated the molecular mechanism of the response of indoleacetic acid (IAA) and cytokinins [zeatin, trans-zeatin riboside (tZR), isopentenyladenine (iP), and isopentenyladenosine (iPA)] to mixed saline-alkali stress (MSAS) in tea crabapple leaves. The endogenous hormone content of tea crabapple leaves under MSAS was measured, and the expression of stress response-related genes was analyzed by RNA sequencing. The results showed that the concentration of IAA was initially higher and then lower than that in the control, whereas the concentration of zeatin, tZR, iP, and iPA was higher than that in the control. A total of 1262 differentially expressed genes were identified in the three comparison groups. Further analyses suggested that IAA and cytokinin biosynthetic genes were mostly upregulated in tea crabapple leaves, indicating that auxin and cytokinin signaling pathway regulation occurred in response to MSAS. These findings suggest that IAA and cytokinins play an important role in the response of tea crabapple to MSAS.
The online version contains supplementary material available at 10.1007/s12298-022-01275-4.
盐碱胁迫是限制全球果树栽培的一种普遍存在的非生物胁迫因素。苹果（×Borkh.）是全球产量最高的水果之一。茶海棠（Rehd. var. Jiang）是中国一种常见的苹果砧木。由于该物种存在兼性无融合生殖现象，因此常用于分子研究。本研究调查了茶海棠叶片中吲哚乙酸（IAA）和细胞分裂素[玉米素、反式玉米素核苷（tZR）、异戊烯腺嘌呤（iP）和异戊烯腺苷（iPA）]对混合盐碱胁迫（MSAS）响应的分子机制。测定了MSAS处理下茶海棠叶片的内源激素含量，并通过RNA测序分析了胁迫响应相关基因的表达。结果表明，IAA浓度最初高于对照，随后低于对照，而玉米素、tZR、iP和iPA的浓度高于对照。在三个比较组中总共鉴定出1262个差异表达基因。进一步分析表明，IAA和细胞分裂素生物合成基因在茶海棠叶片中大多上调，表明生长素和细胞分裂素信号通路调控发生以响应MSAS。这些发现表明，IAA和细胞分裂素在茶海棠对MSAS的响应中起重要作用。
在线版本包含可在10.1007/s12298-022-01275-4获取的补充材料。
