College of Horticulture, Qingdao Agricultural University, Qingdao, 266109, China; Qingdao Key Laboratory of Genetic Improvement and Breeding in Horticulture Plants, Qingdao, 266109, China.
College of Horticulture, Qingdao Agricultural University, Qingdao, 266109, China; Qingdao Key Laboratory of Genetic Improvement and Breeding in Horticulture Plants, Qingdao, 266109, China.
Plant Physiol Biochem. 2021 Jul;164:101-114. doi: 10.1016/j.plaphy.2021.04.022. Epub 2021 May 4.
Apple is among the most widely cultivated perennial fruit crops worldwide. It is sensitive to salt stress, which seriously affects the growth and productivity of apple trees by destroying the homeostasis of Na and K. Previous studies focused on the molecular mechanism underlying NaCl stress. However, signaling transduction under KCl stress has not been thoroughly studied.
We comprehensively analyzed the salt tolerance of Malus hupehensis Rehd., which is a widely used rootstock in apple orchards, by using RNA-Seq. Roots and leaves were treated with NaCl and KCl. Based on mapping analyses, a total of 762 differentially expressed genes (DEGs) related to NaCl and KCl stress in the roots and leaves were identified. Furthermore, we identified seven hub genes by WGCNA Analysis. The Gene Ontology (GO) terms were enriched in ion transmembrane transporter and oxidoreductase activity under NaCl and KCl stress. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways focused on the plant hormone signal transduction and mitogen-activated protein kinase signaling pathway. We also screened out 28 candidate genes from 762 DEGs and verified their expression by quantitative reverse transcription polymerase chain reaction (qRT-PCR). All of these enriched genes were closely related to NaCl and KCl stress and take part in mediating the Na and K homeostasis in M. hupehensis.
This transcriptome analysis provides a valuable resource for elucidating the signaling pathway of NaCl and KCl stress and is a substantial genetic resource for discovering genes related to the NaCl and KCl stress response.
苹果是全球范围内最广泛种植的多年生水果作物之一。它对盐胁迫敏感，通过破坏 Na 和 K 的内稳态严重影响苹果树的生长和生产力。以前的研究集中在 NaCl 胁迫下的分子机制上。然而，KCl 胁迫下的信号转导尚未得到深入研究。
我们通过 RNA-Seq 全面分析了广泛用于苹果园的砧木苹果属华坪瑞木的耐盐性。用 NaCl 和 KCl 处理根和叶。基于映射分析，在根和叶中鉴定出与 NaCl 和 KCl 胁迫相关的总共 762 个差异表达基因（DEGs）。此外，我们通过 WGCNA 分析鉴定出七个枢纽基因。基因本体论（GO）术语在 NaCl 和 KCl 胁迫下富集于离子跨膜转运体和氧化还原酶活性。京都基因与基因组百科全书（KEGG）途径集中在植物激素信号转导和丝裂原激活蛋白激酶信号通路。我们还从 762 个 DEGs 中筛选出 28 个候选基因，并通过定量逆转录聚合酶链反应（qRT-PCR）验证其表达。所有这些富集的基因都与 NaCl 和 KCl 胁迫密切相关，并参与调节 M. hupehensis 的 Na 和 K 内稳态。
该转录组分析为阐明 NaCl 和 KCl 胁迫的信号通路提供了有价值的资源，是发现与 NaCl 和 KCl 胁迫反应相关基因的重要遗传资源。
