Orthopedic Center, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang, China.
Department of Pharmacy, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang, China Rui Tan and Chunbing Zhao contributed equally to this work.
Pol J Pathol. 2022;73(3):264-276. doi: 10.5114/pjp.2022.124493.
Osteosarcoma ranks first in both morbidity and mortality among primary bone tumors. This study aimed to investigate the effect of up-regulation of PRR11 on the malignancy of osteosarcoma and its clinical significance. The expression, biological function, related pathways of PRR11 in osteosarcoma and its impact on prognosis were explored through the bioinformatics database. After PRR11 was up-regulated, cell proliferation, invasion, migration and apoptosis were detected by the cell counting kit-8 method, Transwell, scratch, and flow cytometry. PRR11 is highly expressed in a variety of malignant tumors, including osteosarcoma tissue and cells, and has a significant impact on prognosis. Univariate Cox regression analysis revealed that PRR11 was an independent prognostic factor for osteosarcoma. The gene set enrichment analysis results showed that the differential genes were mainly enriched in the biological process of the cell cycle; the protein-protein interaction network mainly interacted with the regulatory genes of the cell cycle. PRR11 promotes the invasion, migration, and proliferation of osteosarcoma cells and inhibits their apoptosis. Comprehensive bioinformatics analysis revealed that PRR11 promotes the malignancy of osteosarcoma cells mainly by participating in cell cycle regulation, and has an important impact on osteosarcoma prognosis. PRR11 may provide the basis for prognosis and treatment in patients with osteosarcoma.
骨肉瘤在原发性骨肿瘤中发病率和死亡率均居首位。本研究旨在探讨 PRR11 上调对骨肉瘤恶性程度的影响及其临床意义。通过生物信息学数据库探讨 PRR11 在骨肉瘤中的表达、生物学功能、相关通路及其对预后的影响。上调 PRR11 后，通过细胞计数试剂盒-8 法、Transwell、划痕和流式细胞术检测细胞增殖、侵袭、迁移和凋亡。PRR11 在多种恶性肿瘤中高表达，包括骨肉瘤组织和细胞，对预后有显著影响。单因素 Cox 回归分析显示 PRR11 是骨肉瘤的独立预后因素。基因集富集分析结果表明，差异基因主要富集于细胞周期的生物学过程；蛋白质-蛋白质相互作用网络主要与细胞周期的调节基因相互作用。PRR11 促进骨肉瘤细胞的侵袭、迁移和增殖，抑制其凋亡。综合生物信息学分析表明，PRR11 主要通过参与细胞周期调控促进骨肉瘤细胞的恶性程度，对骨肉瘤预后有重要影响。PRR11 可能为骨肉瘤患者的预后和治疗提供依据。
