[电针对慢性非细菌性前列腺炎大鼠前列腺形态及肥大细胞脱颗粒的影响]

[Effect of electroacupuncture on the morphology of prostate and degranulation of mast cells in rats with chronic nonbacterial prostatitis].

作者信息

Li Zhi-Hao, Zhao Xue-Dan, Ming Shu-Ren, Li Xu, Hu Jun-Wei, Chen Yue-Lai

机构信息

Department of Acupuncture-Moxibustion, Tuina and Traumatology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200233, China.

Department of Acupuncture and Moxibustion, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032.

出版信息

Zhen Ci Yan Jiu. 2023 Jan 25;48(1):56-62. doi: 10.13702/j.1000-0607.20211148.

DOI:10.13702/j.1000-0607.20211148

PMID:36734499

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) on the morphology of prostate and degranulation of mast cells in prostate of rats with chronic nonbacterial prostatitis (CNP).

METHODS

Male SD rats were randomly divided into sham operation group, model group and EA group, with 8 rats in each group. CNP model was established by surgical castration combined with 17-β estradiol injection once daily for 30 days. EA was applied to "Zhongji" (CV3), "Guanyuan" (CV4) and bilateral "Dahe" (KI12) for 20 min, once daily for 8 days. The mechanical pain threshold of scrotum skin area was tested before modeling, after modeling and after intervention. The pathological morphology of the prostate was observed by HE staining. Collagenous fiber was observed by Masson staining. The infiltration of mast cells was observed by toluidine blue staining. The contents of interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α) in prostate were determined by ELISA. The protein expression levels of tryptase and transforming growth factor β1 (TGF-β1) in prostate were detected by Western blot.

RESULTS

A normal appearance with little inflammatory cell infiltration was observed in the prostate of the sham operation group. Gland atrophy, epithelial destruction, hyperemia and edema, diffuse inflammatory cell infiltration and visible collagen fiber deposition were observed in prostate of the model group. The degree of infiltration of inflammatory cells and collagen fiber deposition were reduced in the EA group. Compared with the sham operation group, mechanical pain threshold was decreased (<0.01), while the collagen volu-me fraction (CVF) of prostate, the degranulated rate of mast cells, the protein expression levels of tryptase and TGF-β1, and the contents of IL-6 and TNF-α were increased (<0.01) in the model group. Following EA intervention, compared with the model group, the mechanical pain threshold was increased (<0.01), CVF of the prostate, the degranulated rate of mast cells, the protein expression levels of tryptase and TGF-β1, and the contents of IL-6 and TNF-α were decreased (<0.05, <0.01) in the EA group.

CONCLUSION

EA can relieve pain and reduce inflammation and fibrosis of prostate in CNP rats, which may be related to inhibiting the degranulation of prostate mast cells and down-regulating the expression of inflammatory factors and TGF-β1.

摘要

目的

观察电针（EA）对慢性非细菌性前列腺炎（CNP）大鼠前列腺形态及前列腺肥大细胞脱颗粒的影响。

方法

将雄性SD大鼠随机分为假手术组、模型组和电针组，每组8只。采用手术去势并每日注射17-β雌二醇1次，连续30天建立CNP模型。将电针应用于“中极”（CV3）、“关元”（CV4）及双侧“大赫”（KI12），留针20分钟，每日1次，共8天。分别于造模前、造模后及干预后检测阴囊皮肤区域的机械痛阈。采用HE染色观察前列腺的病理形态。采用Masson染色观察胶原纤维。采用甲苯胺蓝染色观察肥大细胞浸润情况。采用ELISA法检测前列腺组织中白细胞介素6（IL-6）和肿瘤坏死因子α（TNF-α）的含量。采用Western blot法检测前列腺组织中类胰蛋白酶和转化生长因子β1（TGF-β1）的蛋白表达水平。

结果

假手术组大鼠前列腺外观正常，炎症细胞浸润少。模型组大鼠前列腺出现腺体萎缩、上皮破坏、充血水肿、弥漫性炎症细胞浸润及可见的胶原纤维沉积。电针组炎症细胞浸润程度及胶原纤维沉积减少。与假手术组比较，模型组大鼠机械痛阈降低（<0.01），前列腺组织胶原容积分数（CVF）、肥大细胞脱颗粒率、类胰蛋白酶和TGF-β1蛋白表达水平及IL-6和TNF-α含量升高（<0.01）。电针干预后，与模型组比较，电针组大鼠机械痛阈升高（<0.01），前列腺组织CVF、肥大细胞脱颗粒率、类胰蛋白酶和TGF-β1蛋白表达水平及IL-6和TNF-α含量降低（<0.05，<0.01）。