Heikal E A, Elamir A M, Hegazi M A, Salem H S, Tawfeik A M, Bosilah A H, Bakry M S, Abdel-Hafez Y N
Department of Medical Parasitology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.
Eur Rev Med Pharmacol Sci. 2023 Jan;27(2):501-510. doi: 10.26355/eurrev_202301_31050.
Infection with Trichomonas vaginalis (TV) is the most prevalent non-viral sexually transmitted infection in the world. The objective of the study was to investigate the incidence of TV infection and its association with Human Papillomavirus (HPV) in a sample of Egyptian females.
96 Egyptian females suspected for trichomoniasis were involved in our study. Vaginal washouts and cervical (cytobrush) samples were obtained from all patients and examined for T. vaginalis by direct wet mount, modified Diamond's culture medium, and real-time PCR. Cervical (cytobrush) samples were examined for HPV using real-time PCR.
Out of 96 patients, 28 (29%) was positive for T. vaginalis by real-time PCR. HPV was detected in 33 patients (34.4%); 31 cases (32.3%) were infected with HPV of genotype 16, whereas only two cases (2.1%) had genotype 18 infection. A significant association was found between TV and HPV infection [Odds ratio (OR)=10.58; 95% confidence interval (CI): 3.819 - 29.29; p<0.001].
When it comes to diagnosing trichomoniasis in a susceptible population, real-time PCR is more reliable than traditional standard approaches. A significant association between TV and HPV infection was found. Further research into the processes by which these two organisms interact at the cellular level could be revealed.
阴道毛滴虫（TV）感染是全球最常见的非病毒性传播感染。本研究的目的是调查埃及女性样本中TV感染的发生率及其与人类乳头瘤病毒（HPV）的关联。
96名疑似滴虫病的埃及女性参与了我们的研究。从所有患者中获取阴道冲洗液和宫颈（细胞刷）样本，通过直接湿片法、改良钻石培养基和实时荧光定量PCR检测阴道毛滴虫。使用实时荧光定量PCR检测宫颈（细胞刷）样本中的HPV。
96名患者中，28例（29%）通过实时荧光定量PCR检测出阴道毛滴虫呈阳性。33例患者（34.4%）检测出HPV；31例（32.3%）感染了16型HPV，而只有2例（2.1%）感染了18型。发现TV与HPV感染之间存在显著关联[比值比（OR）=10.58；95%置信区间（CI）：3.819 - 29.29；p<0.001]。
在易感人群中诊断滴虫病时，实时荧光定量PCR比传统标准方法更可靠。发现TV与HPV感染之间存在显著关联。可能会揭示这两种生物体在细胞水平上相互作用的过程的进一步研究。
