Patra Sumit Kumar, Rabha Monosh, Sen Bhaskar, Aguan Kripamoy, Khatua Snehadrinarayan
Centre for Advanced Studies, Department of Chemistry, North-Eastern Hill University, Shillong, Meghalaya 793022, India.
Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong, Meghalaya 793022, India.
Dalton Trans. 2023 Feb 28;52(9):2592-2602. doi: 10.1039/d2dt03469g.
A red emissive ruthenium(II) complex 1[PF6]2 of an amino ethanol substituted 1,10-phenanthroline-based ligand (L1) has been developed and characterized by spectroscopic analysis and single-crystal X-ray diffraction. Complex 1 shows an aggregation-induced emission (AIE) enhancement and forms nano-aggregates in the poor solvent water and highly dense polyethylene glycol (PEG) media. The possible reason behind the AIE properties may be the rigidity gained through weak supramolecular interactions between neighbouring phenanthroline ligands and PF counterions. The AIE properties were supported by UV-vis and photoluminescence (PL) spectroscopy and dynamic light scattering (DLS) studies to substantiate the formation of nano-aggregates and to understand the morphology of the aggregated particles, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) studies were performed. Compound 1[PF6]2 was highly selective towards pyrophosphate ions (PPi) over other phosphates such as ATP, ADP, AMP and HPO ions and other competitive anions in the PL spectroscopic channel in acetonitrile. The PL titrations of 1[PF6]2 with PPi in CHCN furnished the association constant = 1.08 × 10 M and the detection limit was calculated as low as 1.54 μM. The PPi detection has been established through the unique H-bonding interaction, supported by H NMR titration. Finally, the cytotoxicity study and bioimaging were carried out for biological application. The complex shows very low cytotoxicity and good biocompatibility and is suitable for intracellular PPi imaging.
一种基于氨基乙醇取代的1,10 - 菲咯啉配体（L1）的红色发光钌（II）配合物1[PF6]2已被开发出来，并通过光谱分析和单晶X射线衍射进行了表征。配合物1表现出聚集诱导发光（AIE）增强，并在不良溶剂水和高密度聚乙二醇（PEG）介质中形成纳米聚集体。AIE性质背后的可能原因可能是相邻菲咯啉配体与PF抗衡离子之间通过弱超分子相互作用获得的刚性。通过紫外可见光谱和光致发光（PL）光谱以及动态光散射（DLS）研究支持了AIE性质，以证实纳米聚集体的形成并了解聚集颗粒的形态，还进行了扫描电子显微镜（SEM）和透射电子显微镜（TEM）研究。在乙腈中的PL光谱通道中，化合物1[PF6]2对焦磷酸根离子（PPi）的选择性高于其他磷酸盐，如ATP、ADP、AMP和HPO离子以及其他竞争性阴离子。1[PF6]2与PPi在CHCN中的PL滴定给出了缔合常数 = 1.08 × 10 M，检测限计算低至1.54 μM。通过独特的氢键相互作用建立了PPi检测，1H NMR滴定提供了支持。最后，进行了细胞毒性研究和生物成像以用于生物应用。该配合物显示出非常低的细胞毒性和良好的生物相容性，适用于细胞内PPi成像。
