1The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; 2Shanghai Municipal Key Clinical Specialty, Shanghai, China.
1The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; 2Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, China.
Acta Biochim Pol. 2023 Feb 3;70(1):131-135. doi: 10.18388/abp.2020_6331.
To investigate the effects of resveratrol on multiple organ injury and energy metabolism and its possible mechanism in preeclampsia model.
A total of 45 clean-grade female adults Sprague Dawley (SD) rats (weight 270-320 g) were randomly divided into three groups: a control group, preeclampsia group (PE), and Preeclampsia with resveratrol treatment group (RE). Preeclampsia was induced in rats by administering 200 mg/kg/day L-NAME. Expression levels of TNF-α and IL-6 in the lungs and kidney tissues were analysed by ELISA, while the activity of superoxide dismutase (SOD) and malondialdehyde (MDA) was determined by biochemical assays. The levels of lactic acid and pyruvate were detected using biochemical assays, while the epinephrine level in the kidney and heart tissues was determined by the ELISA method. Reverse transcription polymerase chain reaction and the western blotting were used to detect the expression of pyruvate dehydrogenase kinase 4 (PDK4) in the myocardial tissues.
We found that resveratrol treatment inhibited the levels of IL-6, TNF-α, and MDA in the lungs and kidney tissues, while the SOD activity was increased. Treatment with resveratrol reduced the levels of lactic acid, pyruvate, and epinephrine in the kidney and heart tissues. Furthermore, resveratrol treatment significantly increases the expression of PDK4 myocardial tissues in RE group compared to PE group.
Resveratrol may inhibit the release of tissue inflammatory factors, regulate the body's energy metabolism, and ultimately protect tissue damage caused during Preeclampsia.
探讨白藜芦醇对子痫前期模型多器官损伤及能量代谢的影响及其可能机制。
将 45 只清洁级成年 SD 大鼠（体重 270-320g）随机分为 3 组：对照组、子痫前期组（PE）和子痫前期白藜芦醇治疗组（RE）。采用 200mg/kg/d L-NAME 诱导大鼠子痫前期。采用 ELISA 法检测肺、肾组织中 TNF-α、IL-6 的表达水平，生化法检测超氧化物歧化酶（SOD）和丙二醛（MDA）的活性。采用生化法检测乳酸和丙酮酸的水平，采用 ELISA 法检测肾、心组织中肾上腺素的水平。采用逆转录聚合酶链反应和蛋白质印迹法检测心肌组织中丙酮酸脱氢酶激酶 4（PDK4）的表达。
白藜芦醇治疗组抑制了肺、肾组织中 IL-6、TNF-α 和 MDA 的水平，同时增加了 SOD 活性。白藜芦醇治疗组降低了肾、心组织中乳酸、丙酮酸和肾上腺素的水平。此外，与 PE 组相比，RE 组心肌组织中 PDK4 的表达明显增加。
白藜芦醇可能通过抑制组织炎症因子的释放，调节机体能量代谢，从而保护子痫前期引起的组织损伤。
