Kerdsuknirund Sasitorn, Thaeomor Atcharaporn, Kupittayanant Pakanit, Khunkaewla Panida, Chanlun Suthida, Srisawat Rungrudee, Tongdee Pattama, Nimkuntod Porntip, Kupittayanant Sajeera
School of Preclinical Sciences, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Nutrients. 2025 Mar 26;17(7):1143. doi: 10.3390/nu17071143.
: This study aimed to evaluate the dose-dependent therapeutic effects of pomegranate juice on preeclampsia symptoms using an L-NAME-induced rat model. : Pregnant rats ( = 5/group) were assigned to a negative control group or groups receiving L-NAME to induce preeclampsia, with pomegranate juice administered at low, medium, and high doses from gestation day 7 to 20. Maternal parameters, including body weight, systolic blood pressure, urinary protein, and sFlt-1 levels, were monitored. Kidney and placental histology were assessed on gestation day 20. : L-NAME successfully induced preeclampsia symptoms, including significant maternal weight gain, hypertension, proteinuria, and increased sFlt-1 levels. Pomegranate juice administration alleviated these symptoms in a dose-dependent manner. High doses significantly prevented weight gain from gestation day 14, reduced the systolic blood pressure from gestation day 16, and lowered proteinuria and the sFlt-1 levels by gestation day 18, achieving values comparable to those of normal pregnant controls. Medium doses showed a moderate improvement, particularly in later gestational stages, while low doses had minimal effects. Pomegranate juice also enhanced placental health by increasing the labyrinth depth and reducing endocapillary hypercellularity, contributing to higher fetal and placental birth weights. The dose-response analysis indicated that the kidneys exhibited a stronger response to pomegranate juice than the placenta, suggesting different sensitivity thresholds. : Pomegranate juice alleviates preeclampsia symptoms in a dose-dependent manner, significantly improving maternal weight regulation, blood pressure, and proteinuria. The therapeutic effects of pomegranate juice are attributed to its high phenolic content, which reduces sFlt-1 and improves placental function. These findings support pomegranate juice as a potential natural intervention for preeclampsia management.
本研究旨在利用L-精氨酸甲酯（L-NAME）诱导的大鼠模型，评估石榴汁对先兆子痫症状的剂量依赖性治疗效果。将怀孕大鼠（每组n = 5）分为阴性对照组或接受L-NAME诱导先兆子痫的组，从妊娠第7天至第20天给予低、中、高剂量的石榴汁。监测母体参数，包括体重、收缩压、尿蛋白和可溶性血管内皮生长因子受体-1（sFlt-1）水平。在妊娠第20天评估肾脏和胎盘组织学。L-NAME成功诱导了先兆子痫症状，包括母体体重显著增加、高血压、蛋白尿和sFlt-1水平升高。给予石榴汁以剂量依赖性方式缓解了这些症状。高剂量在妊娠第14天显著阻止体重增加，在妊娠第16天降低收缩压，在妊娠第18天降低蛋白尿和sFlt-1水平，达到与正常怀孕对照组相当的值。中等剂量显示出适度改善，特别是在妊娠后期，而低剂量效果最小。石榴汁还通过增加迷路深度和减少毛细血管内细胞增多来改善胎盘健康，有助于提高胎儿和胎盘出生体重。剂量反应分析表明，肾脏对石榴汁的反应比胎盘更强，表明存在不同的敏感性阈值。石榴汁以剂量依赖性方式缓解先兆子痫症状，显著改善母体体重调节、血压和蛋白尿。石榴汁的治疗效果归因于其高酚含量，可降低sFlt-1并改善胎盘功能。这些发现支持石榴汁作为先兆子痫管理的潜在天然干预措施。
