Isoda K, Maeda T, Hamamoto Y
Department of Pathology, Osaka Medical College, Japan.
Acta Pathol Jpn. 1987 Aug;37(8):1305-18. doi: 10.1111/j.1440-1827.1987.tb00463.x.
By the microautoradiographic method using 3H-proline, collagen production by mesothelial cells was investigated in adriamycin-induced pleuritis in rats. In subpleural granulation tissue formed at 4 to 7 days after the intrapleural injection of adriamycin, proliferating fibroblasts and primitive mesenchymal cells were most intensely labeled, and abundant deposition of collagen and acid mucopolysaccharides were demonstrated about these cells. It is thus concluded that these subpleural mesenchymal cells are mainly responsible for the fibrosing process. Labeling was also observed in some reactive mesothelial cells and macrophages free-floating in the pleural exudate. Several ultrastructural differences between labeled mesothelial cells floating in the fluid and lining the pleural surface were confirmed, suggesting a change in ability to synthesize collagen during mesothelial desquamation. It seems likely that these labeled mononuclear cells in the effusion, attached to the pleural wound surface, support fibrosis performed by underlying collagen-synthesizing mesenchymal cells. Pleural fibrosis disappeared by 10 days, when mesothelial regeneration was almost complete. Probably this change may be due to fibrinolytic activity caused by regenerative mesothelial cells derived from subpleural mesenchymal cells.
采用³H - 脯氨酸的微量放射自显影法，研究了大鼠阿霉素诱导性胸膜炎中间皮细胞的胶原生成情况。在胸腔内注射阿霉素后4至7天形成的胸膜下肉芽组织中，增殖的成纤维细胞和原始间充质细胞标记最为强烈，并且在这些细胞周围显示出大量的胶原和酸性粘多糖沉积。因此得出结论，这些胸膜下间充质细胞是纤维化过程的主要责任者。在一些反应性间皮细胞和游离于胸腔渗出液中的巨噬细胞中也观察到了标记。证实了漂浮在液体中并衬于胸膜表面的标记间皮细胞之间存在一些超微结构差异，提示间皮脱屑过程中胶原合成能力发生了变化。似乎积液中这些附着于胸膜创面的标记单核细胞，支持了由其下方胶原合成间充质细胞进行的纤维化。当间皮再生几乎完成时，胸膜纤维化在10天时消失。这种变化可能是由于源自胸膜下间充质细胞的再生间皮细胞引起的纤溶活性所致。
