Sun Jing, Liu Hong, Yao Xin-Yan, Zhang Yu-Qian, Lv Zhi-Hang, Shao Jian-Wei
School of Life Science and Engineering, Foshan University, Foshan, Guangdong Province, China.
School of Life Science and Engineering, Foshan University, Foshan, Guangdong Province, China.
Ticks Tick Borne Dis. 2023 May;14(3):102136. doi: 10.1016/j.ttbdis.2023.102136. Epub 2023 Feb 1.
Ticks play an important role in the evolution and transmission of Anaplasmataceae bacteria which are agents of emerging infectious diseases. In this study, a total of 1286 adult ticks belonging to five species were collected from cattle, goats, horses and vegetation in Harbin area, Heilongjiang province, northeastern China. The tick-borne Anaplasmataceae bacteria were identified by amplifying and sequencing the 16S rRNA (rrs) and heat shock protein-60 encoding (groEL) genes. The results showed that Ixodes persulcatus was dominant (38.8%, 499/1283) among the five tick species, and Anaplasmataceae bacteria were detected in all tick species with an overall prevalence of 7.4%. Four species of Anaplasmataceae bacteria (Anaplasma phagocytophilum, Anaplasma ovis, Anaplasma bovis, and "Candidatus Neoehrlichia mikurensis"), which are pathogenic to humans and/or animals, were identified from tick samples by phylogenetic analyzes of the rrs and groEL gene sequences. Interestingly, the cluster 1 strains were first identified in Asian, and a novel cluster was also detected in this study. These data revealed the genetic diversity of Anaplasmataceae bacteria circulating in ticks in Harbin area, highlighting the need to investigate these tick-borne pathogens and their risks to human and animal health.
蜱虫在无形体科细菌的进化和传播中起着重要作用，这些细菌是新兴传染病的病原体。在本研究中，从中国东北黑龙江省哈尔滨市的牛、山羊、马和植被中总共采集了1286只属于五个物种的成年蜱虫。通过对16S rRNA（rrs）和编码热休克蛋白60（groEL）的基因进行扩增和测序，鉴定蜱传无形体科细菌。结果表明，全沟硬蜱在五个蜱种中占主导地位（38.8%，499/1283），并且在所有蜱种中均检测到无形体科细菌，总体患病率为7.4%。通过对rrs和groEL基因序列进行系统发育分析，从蜱虫样本中鉴定出四种对人类和/或动物致病的无形体科细菌（嗜吞噬细胞无形体、绵羊无形体、牛无形体和“新立克次体密库菌”）。有趣的是，1型菌株首次在亚洲被鉴定出来，并且在本研究中还检测到一个新的菌株群。这些数据揭示了哈尔滨地区蜱虫中传播的无形体科细菌的遗传多样性，突出了调查这些蜱传病原体及其对人类和动物健康风险的必要性。
