Lu Miao, Tian Jun-Hua, Yu Bin, Guo Wen-Ping, Holmes Edward C, Zhang Yong-Zhen
State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Wuhan Center for Disease Control and Prevention, Wuhan, Hubei Province, China.
Ticks Tick Borne Dis. 2017 Jun;8(4):574-580. doi: 10.1016/j.ttbdis.2017.03.006. Epub 2017 Mar 24.
Rickettsiales bacteria are important agents of (re)emerging infectious diseases, with ticks playing a key role in their evolution and transmission. We collected 1079 hard ticks belonging to five species (Ixodes sinensis, Rhipicephalus microplus, Haemaphysalis flava, Haemaphysalis hystricis and Haemaphysalis longicornis) from cattle and goats in Wuhan city, Hubei province, China. The dominant tick species was H. longicornis (578, 53.57%), followed by R. microplus (354, 32.81%), H. hystricis (62, 5.75%), H. flava (57, 5.28%), and I. sinensis (28, 2.59%). Rickettsiales bacteria were identified in these ticks by amplifying the Rickettsiales 16S rRNA (rrs), citrate synthase (gltA), and heat shock protein (groEL) genes. The rrs gene of Rickettsiales was positive in 32 (2.97%) ticks, including 2 cases of co-infection, with 4 (0.69%) in H. longicornis, 15 (4.24%) in R. microplus, 7 (12.28%) in H. flava, 1 (1.61%) in H. hystricis, and 5 (17.86%) in I. sinensis ticks. Phylogenetic analysis revealed the presence of six recognized and seven Candidatus species of Rickettsiaceae, Anaplasmataceae and Candidatus Midichloriaceae. Notably, one lineage within both Ehrlichia and Candidatus Midichloriaceae was distinct from any known Rickettsiales, suggesting the presence of potentially novel species of Rickettsiales bacteria. In sum, these data reveal an extensive diversity of Rickettsiales in ticks from Wuhan, highlighting the need to understand Rickettsiales infection in local animals and humans.
立克次氏体细菌是（再）新兴传染病的重要病原体，蜱在其进化和传播中起着关键作用。我们从中国湖北省武汉市的牛和山羊身上采集了1079只硬蜱，分属于五个物种（中华硬蜱、微小牛蜱、黄褐血蜱、刺猬血蜱和长角血蜱）。优势蜱种是长角血蜱（578只，占53.57%），其次是微小牛蜱（354只，占32.81%）、刺猬血蜱（62只，占5.75%）、黄褐血蜱（57只，占5.28%）和中华硬蜱（28只，占2.59%）。通过扩增立克次氏体16S rRNA（rrs）、柠檬酸合酶（gltA）和热休克蛋白（groEL）基因，在这些蜱中鉴定出立克次氏体细菌。立克次氏体的rrs基因在32只（2.97%）蜱中呈阳性，包括2例合并感染，其中长角血蜱中有4只（0.69%）、微小牛蜱中有15只（4.24%）、黄褐血蜱中有7只（12.28%）、刺猬血蜱中有1只（1.61%）、中华硬蜱中有5只（17.86%）。系统发育分析显示存在立克次体科、无形体科和候选米氏菌科的6个已确认物种和7个候选物种。值得注意的是，埃立克体属和候选米氏菌科中的一个谱系与任何已知的立克次氏体不同，这表明存在潜在的新型立克次氏体细菌物种。总之，这些数据揭示了武汉蜱中立克次氏体的广泛多样性，突出了了解当地动物和人类中立克次氏体感染情况的必要性。
