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采用气相色谱-质谱联用技术检测和分类三种真菌感染柑橘果实释放的挥发性化合物。

Detection and classification of volatile compounds emitted by three fungi-infected citrus fruit using gas chromatography-mass spectrometry.

机构信息

Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, PR China; Horticultural Products Cold Chain Logistics Technology and Equipment National-Local Joint Engineering Laboratory, Hangzhou 310058, PR China; Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Zhejiang University, Hangzhou 310058, PR China.

College of Animal Sciences, Zhejiang University, Zijingang Campus, Hangzhou 310058, PR China.

出版信息

Food Chem. 2023 Jun 30;412:135524. doi: 10.1016/j.foodchem.2023.135524. Epub 2023 Jan 20.

Abstract

Citrus fruit produced some characteristic volatile compounds when infected by fungi compared with the healthy fruit. In the present study, volatile metabolites of postharvest citrus fruit with three different diseases including stem-end rot, blue mold and green mold were detected. Multivariate analysis such as principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were employed to classify the volatile compounds between the infected and non-infected citrus fruit. The results indicated that volatile compounds of unrotten, unrotten-rotten junction, and rotten tissues were successfully classified. Importantly, eight volatile compounds as biomarkers for stem-end rot and one biomarker for green mold of citrus were screened to discriminate the infected citrus fruit. This study offers the application potential of odor profiling of volatile compounds for detecting the fungi infection in postharvest citrus fruit.

摘要

与健康水果相比,感染真菌的柑橘类水果会产生一些特征性的挥发性化合物。在本研究中,检测了三种不同疾病(包括蒂腐病、青霉病和绿霉病)的采后柑橘果实的挥发性代谢物。采用主成分分析(PCA)和偏最小二乘判别分析(PLS-DA)等多元分析方法对感染和未感染柑橘果实之间的挥发性化合物进行分类。结果表明,未腐烂、未腐烂-腐烂交界处和腐烂组织的挥发性化合物可以成功分类。重要的是,筛选出了 8 种作为柑橘蒂腐病生物标志物和 1 种作为柑橘绿霉病生物标志物的挥发性化合物,可用于区分感染的柑橘果实。本研究为利用挥发性化合物气味特征分析检测采后柑橘果实真菌感染提供了应用潜力。

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