CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, 730000, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China.
CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, 730000, PR China.
Talanta. 2023 May 1;256:124322. doi: 10.1016/j.talanta.2023.124322. Epub 2023 Jan 31.
Herein, we developed a class of functionalized silicon nanoparticles (F-SiNPs) bio-probes named thiol-conjugated F-SiNPs. They combine excellent biocompatibility with small dimensions (<10 nm) and biological usefulness with sustained and robust fluorescence (3.32% photoluminescent quantum yield). Identifying 3-Mercaptopropionic acid (3-MPA), which lowers the quantity of gamma-aminobutyric acid in the brain, and mercury (Hg) was a crucially important step since their excessive levels are a sign of several disorders. Using F-SiNPs as a fluorescent bio-probe, we provided an "off-on" technique for sensitively and selectively determining Hg and 3-MPA in this study. The 3-(2-aminoethylamino) propyl (dimethoxymethylsilane) and basic fuchsin as precursors were hydrothermally treated to produce the F-SiNPs exhibiting green fluorescence. Our results suggest that Hg reduced the fluorescence of F-SiNPs because of strong ionic interactions and metal-ligand binding among many thiols and carboxyl groupings at the surface of Hg and F-SiNPs. Additionally, the resultants demonstrated that after being quenched by Hg, the produced F-SiNPs led to the distinctive "off-on" response to 3-MPA. Moreover, the method could detect Hg and 3-MPA with limits of detection of 0.065 μM and 0.017 μM, respectively. The technique employed is quick, easy, affordable, and environmentally friendly. The sensing platform has successfully determined Hg and 3-MPA in urine, water, and human serum samples.
在此，我们开发了一类名为巯基修饰的硅纳米粒子（F-SiNPs）的功能化硅纳米粒子生物探针。它们结合了优异的生物相容性、小尺寸（<10nm）和生物利用度以及持续且强的荧光（3.32%的光致发光量子产率）。鉴定 3-巯基丙酸（3-MPA）和汞（Hg）是至关重要的一步，因为它们的过量水平是几种疾病的标志。在这项研究中，我们使用 F-SiNPs 作为荧光生物探针，提供了一种“关闭-开启”技术，用于灵敏和选择性地测定 Hg 和 3-MPA。3-（2-氨乙基氨基）丙基（二甲氧基甲基硅烷）和碱性品红作为前体，经过水热处理，生成了发出绿色荧光的 F-SiNPs。我们的结果表明，Hg 由于其表面上的许多巯基和羧基基团之间的强离子相互作用和金属配体结合，降低了 F-SiNPs 的荧光。此外，结果表明，被 Hg 猝灭后，生成的 F-SiNPs 对 3-MPA 产生了独特的“关闭-开启”响应。此外，该方法可以检测 Hg 和 3-MPA，检测限分别为 0.065μM 和 0.017μM。该技术快速、简单、经济实惠且环保。传感平台已成功测定了尿液、水和人血清样品中的 Hg 和 3-MPA。
