School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China.
Henan Engineering Research Center of Modern Chinese Medicine Research, Development and Application, Zhengzhou, 450046, China.
Anal Methods. 2023 Jun 29;15(25):3034-3042. doi: 10.1039/d3ay00421j.
Bilirubin, a tetrapyrrole compound metabolized by heme, is an important biomarker for diagnosis and prognosis of patients with liver diseases. Highly sensitive detection of bilirubin is essential for disease prevention and treatment. In recent years, silicon nanoparticles (SiNPs) have received intense attention due to their excellent optical properties and environmental friendliness. In this paper, water-soluble yellow-green fluorescent SiNPs were synthesized by a mild water bath method using 2-aminophenylboronic acid hydrochloride as the reducing agent and 3-[2-(2-aminoethylamino)ethylamino]propyl-trimethoxysilane (AEEA) as the silicon source. The preparation process does not require high temperature, high pressure and complex modifications. The SiNPs possessed excellent photostability and good water dispersibility. It was found that the fluorescence of SiNPs at 536 nm could be significantly quenched by bilirubin. By using SiNPs as a fluorescent probe, a novel fluorescence method for sensitive detection of bilirubin was established with a wide linear range of 0.05-75 μM and a limit of detection (LOD) of 16.67 nM. The detection mechanism was mainly due to the internal filtration effect (IFE). More significantly, the established method could successfully determine the contents of bilirubin in biological samples with good recoveries.
胆红素是血红素代谢产生的四吡咯化合物，是诊断和预测肝脏疾病患者病情的重要生物标志物。胆红素的高灵敏度检测对于疾病的预防和治疗至关重要。近年来，由于硅纳米粒子（SiNPs）具有优异的光学性能和环境友好性，因此受到了广泛关注。本文采用温和的水热法，以 2-氨基苯硼酸盐酸盐为还原剂，3-[2-(2-氨乙基氨基)乙基氨基]丙基三甲氧基硅烷（AEEA）为硅源，合成了水溶性的黄绿色荧光 SiNPs。该制备过程不需要高温、高压和复杂的修饰。SiNPs 具有良好的光稳定性和良好的水分散性。研究发现，SiNPs 在 536nm 处的荧光可以被胆红素显著猝灭。利用 SiNPs 作为荧光探针，建立了一种用于灵敏检测胆红素的新型荧光方法，其线性范围为 0.05-75μM，检测限（LOD）为 16.67nM。检测机制主要归因于内滤效应（IFE）。更重要的是，该方法可以成功地测定生物样品中胆红素的含量，回收率良好。
