Miele Dalila, Nomicisio Cristian, Musitelli Giorgio, Boselli Cinzia, Icaro Cornaglia Antonia, Sànchez-Espejo Rita, Vigani Barbara, Viseras Cesar, Rossi Silvia, Sandri Giuseppina
Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.
Department of Public Health, Experimental and Forensic Medicine, University of Pavia, via Forlanini 2, 27100 Pavia, Italy.
Int J Pharm. 2023 Mar 5;634:122669. doi: 10.1016/j.ijpharm.2023.122669. Epub 2023 Feb 1.
Fiber spinning technologies attracted a great interest since the beginning of the last century. Among these, electrospinning is a widely diffuse technique; however, it presents some drawbacks such as low fiber yield, high energy demand and the use of organic solvents. On the contrary, centrifugal spinning is a more sustainable method and allows to obtain fiber using centrifugal force and melted materials. The aim of the present work was the design and the development of polydioxanone (PDO) microfibers intended for tissue engineering, using centrifugal spinning. PDO, a bioresorbable polymer currently used for sutures, was selected as low melting polyester and DES (deep eutectic solvents), either choline chloride/citric acid (ChCl/CA) or betaine/citric acid (Bet/CA) 1:1 M ratio, were used to improve PDO spinnability. Physical mixtures of DES and PDO were prepared using different weight ratios. These were then poured into the spinneret and melted at 140 °C for 5 min. After the complete melting, the blends were spun for 1 min at 700 rpm. The fibers were characterized for physico chemical properties (morphology; dimensions; chemical structure; thermal behavior; mechanical properties). Moreover, the preclinical investigation was performed in vitro (biocompatibility, adhesion and proliferation of fibroblasts) and in vivo (murine burn/excisional model to assess safety and efficacy). The multidisciplinary approach allowed to obtain an extensive characterization to develop PDO based microfibers as medical device for implant to treat full thickness skin wounds.
自上世纪初以来，纤维纺丝技术就引起了人们的极大兴趣。其中，静电纺丝是一种广泛应用的技术；然而，它存在一些缺点，如纤维产量低、能源需求高以及使用有机溶剂。相反，离心纺丝是一种更具可持续性的方法，它利用离心力和熔融材料来制备纤维。本研究的目的是利用离心纺丝技术设计和开发用于组织工程的聚对二氧环己酮（PDO）微纤维。PDO是一种目前用于缝合线的生物可吸收聚合物，被选为低熔点聚酯，并使用1:1摩尔比的氯化胆碱/柠檬酸（ChCl/CA）或甜菜碱/柠檬酸（Bet/CA）等低共熔溶剂（DES）来提高PDO的可纺性。使用不同的重量比制备了DES和PDO的物理混合物。然后将这些混合物倒入喷丝头中，在140℃下熔融5分钟。完全熔融后，将共混物以700转/分钟的速度纺丝1分钟。对纤维的物理化学性质（形态、尺寸、化学结构、热行为、机械性能）进行了表征。此外，还进行了临床前体外研究（成纤维细胞的生物相容性、粘附和增殖）和体内研究（小鼠烧伤/切除模型，以评估安全性和有效性）。这种多学科方法能够获得广泛的表征，从而开发出基于PDO的微纤维作为用于治疗全层皮肤伤口的植入医疗器械。
