微光度法测定叠连红细胞内氧饱和度的变异性。
The microphotometric determination of the variability of oxygen saturation of erythrocytes lying within rouleaux.
作者信息
Lubbers D W, Grisar H, Gayeski T E
机构信息
Max-Planck-Institut fur Systemphysiologie, Dortmund, F.R.G.
出版信息
Adv Exp Med Biol. 1987;215:35-43. doi: 10.1007/978-1-4684-7433-6_5.
It was found that by using nonlinear multicomponent analysis with the spectra of oxygenated and deoxygenated haemoglobin as basic spectra the mean O2 saturation of 2 to 3 red cells lying in a rouleau could be determined with an accuracy of 0.5 - 1.0%. At the same PO2 and PCO2 distinct differences in O2 saturation were found in different red cells; for 50% of the saturation values the differences were in the range +/- 1.5%, for 30% of the values, the differences were in the range of +/- 3% and 15% lay in the range of +/- 4.5%. Only 5% of saturation differences were larger than 4.6%. These differences are so large that they have to be considered in calculations of the PO2 from O2 saturation measurements.
研究发现，以氧合血红蛋白和脱氧血红蛋白的光谱作为基础光谱，通过非线性多组分分析，可以测定叠连排列的2至3个红细胞的平均氧饱和度，其准确度可达0.5 - 1.0%。在相同的氧分压（PO2）和二氧化碳分压（PCO2）条件下，不同红细胞的氧饱和度存在明显差异；50%的饱和度值差异在±1.5%范围内，30%的饱和度值差异在±3%范围内，15%的饱和度值差异在±4.5%范围内。只有5%的饱和度差异大于4.6%。这些差异非常大，因此在根据氧饱和度测量值计算氧分压时必须予以考虑。
Zotero 插件