Laboratory of Biotechnology, Chemistry and Food School, Federal University of Rio Grande, Rio Grande, RS, 96203-900, Brazil.
Landscape, Environment, Agriculture and Food - LEAF Centre, Instituto Superior de Agronomia, Universidade de Lisboa, 1349-017, Lisboa, Portugal.
Curr Microbiol. 2023 Feb 4;80(3):96. doi: 10.1007/s00284-023-03189-0.
The application of magnetic fields (MF) has attracted the attention of researchers due to their efficiency to change cell metabolism. Chlamydomonas reinhardtii is a biotechnologically useful microalga with versatile metabolism that may be a valuable organism to study the effects of the MF in biology. Therefore, two C. reinhardtii strains, one with cell wall (2137) and other which lacks the cell wall (Wt-S1-cc4694), were evaluated that a new sensitivity factor in the analysis could be included. Comparative studies were undertaken with the two C. reinhardtii strains under the MF intensities of 0.005 mT (terrestrial MF - control), 11 and 20 mT. Results indicated that the physical cell wall barrier protected cells against the MF applied during the assays. Only with the highest MF applied (20 mT) a slight increase in lipid concentration in the cell wall strain was detected. The lowest growth of the strain that lacks cell wall (Wt-S1) indicated that these cells are under a negative effect. To cope with the two MF stresses conditions, Wt-S1 cells produced more pigments (chlorophylls and carotenoids) and lipids and enhanced the antioxidant defense system. The raise of these compounds under MF could potentially have a positive biotechnological impact on algal biomass.
磁场（MF）的应用由于其改变细胞代谢的效率而引起了研究人员的关注。莱茵衣藻是一种具有多功能代谢的生物技术有用的微藻，可能是研究 MF 对生物学影响的有价值的生物体。因此，评估了两种具有细胞壁的衣藻（2137）和另一种缺乏细胞壁的衣藻（Wt-S1-cc4694），可以在分析中包括一个新的敏感因子。对这两种衣藻菌株在 0.005 mT（地磁场-对照）、11 mT 和 20 mT 的 MF 强度下进行了比较研究。结果表明，物理细胞壁屏障保护细胞免受实验过程中施加的 MF。只有在施加最高 MF（20 mT）时，才检测到细胞壁菌株中脂质浓度略有增加。缺乏细胞壁的菌株（Wt-S1）的最低生长表明这些细胞受到负面影响。为了应对两种 MF 应激条件，Wt-S1 细胞产生了更多的色素（叶绿素和类胡萝卜素）和脂质，并增强了抗氧化防御系统。MF 下这些化合物的增加可能对藻类生物质具有积极的生物技术影响。
