State Key Laboratory of Organic-Inorganic Composites, National Energy Research and Development Center for Biorefinery, Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China.
Clinical Laboratory, China-Japan Friendship Hospital, Beijing, 100029, China.
Adv Healthc Mater. 2023 May;12(13):e2300146. doi: 10.1002/adhm.202300146. Epub 2023 Feb 3.
A new recognition method is explored for the rapid detection of B-type natriuretic peptide (BNP) based on the rational design and solid-phase synthesis of molecularly imprinted nanoparticles (nanoMIP) encapsulated with carbon dots. The nanosized magnetic template is first prepared by attaching the epitope of BNP on amino-functionalized magnetic carriers. High-dilution polymerization of monomers in the presence of magnetic template generates lightly crosslinked imprinted nanoparticles. To obtain the optimal MIP formulation, a new combinatorial screening approach is developed by a competitive fluorescence assay using the magnetic template. The resultant nanoMIP exhibits high affinity and selectivity toward BNP with an equilibrium dissociation constant (K ) of ≈10 m. The proposed assay allows fast BNP detection within ≈7 min with a linear range of its concentration from 0.25 to 5000 pg mL and a limit of detection of 0.208 pg mL (S/N = 3). To demonstrate its practicability in clinical diagnosis, unknown real serum samples from 160 individuals are analyzed and the relative standard deviation is less than 4.43%. Compared with the routine electrochemiluminescence detection method that is widely used in hospital, the relative error is less than 4.98% and the correlation coefficient is 0.994.
一种新的识别方法是探索基于合理设计和固相合成的分子印迹纳米粒子（nanoMIP）封装的碳点的 B 型利钠肽（BNP）的快速检测。首先通过将 BNP 的表位附着在氨基功能化磁性载体上制备纳米尺寸的磁性模板。在磁性模板存在下，单体的高稀释聚合生成轻度交联的印迹纳米粒子。为了获得最佳的 MIP 配方，通过使用磁性模板的竞争性荧光测定开发了一种新的组合筛选方法。所得的 nanoMIP 对 BNP 表现出高亲和力和选择性，平衡解离常数（K ）约为 10 毫摩尔。该测定法允许在 ≈7 分钟内快速检测 BNP，其浓度线性范围为 0.25 至 5000 pg mL，检测限为 0.208 pg mL（S/N = 3）。为了证明其在临床诊断中的实用性，分析了来自 160 个人的未知真实血清样本，相对标准偏差小于 4.43%。与医院广泛使用的常规电化学发光检测方法相比，相对误差小于 4.98%，相关系数为 0.994。
