Xu Haishan, Fu Xincheng, Kong Hui, Chen Fei, Chang Xia, Ding Zemin, Wang Rongrong, Shan Yang, Ding Shenghua
Longping Branch, College of Biology, Hunan University, Changsha 410125, China; Hunan Agricultural Product Processing Institute, Hunan Academy of Agricultural Sciences, Hunan Provincial Key Laboratory for Fruits and Vegetables Storage Processing and Quality Safety, Changsha 410125, China.
College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China.
Food Res Int. 2023 Feb;164:112327. doi: 10.1016/j.foodres.2022.112327. Epub 2022 Dec 19.
Ultrasonication (US)-assisted Fenton-system (US-Fenton) with different US time was developed for synthesizing chitosan (CS)-ferulic acid (FA) conjugates. The optimal US-Fenton for a suitable time was selected for preparing a film with CS-FA conjugate and its structural, functional, rheological, and physical properties were also investigated. Compared with Fenton-system, US-Fenton enhanced the grafting ratio of the conjugates, which increased firstly and then decreased as US time. The conjugate obtained by US-Fenton for 1 min (FUS1) possessed the highest grafting ratio (121.28 mg FA/g) and its grafting time was also shortened from 12 h to 1 min contrasted with Fenton grafted method. Structural characterization results showed that FA was conjugated on CS via ester and amide bonds with decreased crystallinity. Scanning electron microscopy and molecular weight analysis indicated that the degradation degree of CS-FA conjugates increased with US time. The DPPH and ABTS radical-scavenging activities of FUS1 were the closest to ascorbic acid, and it also showed the best antibacterial effect among the test conjugates. Accordingly, FUS1 was selected to obtain the film for contrasting with CS film. FUS1 film solution exhibited a decreased viscosity. In comparison to CS film, UV transmittance of FUS1 film approached zero, and its moisture, oxygen, and carbon dioxide permeabilities significantly decreased (P < 0.05). Moreover, its water solubility and tensile strength increased by 58.09% and 25.72% than those of CS film, respectively. Therefore, US-Fenton for 1 min could be a promising method for efficiently preparing active food package materials and FUS1 film possessed broad application prospects.
开发了具有不同超声时间的超声（US）辅助芬顿体系（US-Fenton）用于合成壳聚糖（CS）-阿魏酸（FA）共轭物。选择合适时间的最佳US-Fenton来制备含有CS-FA共轭物的薄膜，并对其结构、功能、流变学和物理性质进行了研究。与芬顿体系相比，US-Fenton提高了共轭物的接枝率，接枝率随超声时间先增加后降低。超声处理1分钟的US-Fenton法得到的共轭物（FUS1）具有最高的接枝率（121.28mg FA/g），与芬顿接枝法相比，其接枝时间也从12小时缩短至1分钟。结构表征结果表明，FA通过酯键和酰胺键与CS共轭，结晶度降低。扫描电子显微镜和分子量分析表明，CS-FA共轭物的降解程度随超声时间增加。FUS1的DPPH和ABTS自由基清除活性最接近抗坏血酸，在测试的共轭物中也表现出最佳的抗菌效果。因此，选择FUS1来制备薄膜与CS薄膜进行对比。FUS1薄膜溶液的粘度降低。与CS薄膜相比，FUS1薄膜的紫外线透过率接近零，其水分、氧气和二氧化碳渗透率显著降低（P<0.05）。此外，其水溶性和拉伸强度分别比CS薄膜提高了58.09%和25.72%。因此，超声处理1分钟的US-Fenton可能是一种高效制备活性食品包装材料的有前景的方法，FUS1薄膜具有广阔的应用前景。
