Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, China.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, China.
Int J Biol Macromol. 2022 Jun 15;210:33-43. doi: 10.1016/j.ijbiomac.2022.05.010. Epub 2022 May 6.
Anthocyanins (ACNs) have attracted considerable research attention because of their excellent health benefits, but their low stability and bioavailability limit their applications. In this study, sinapic acid-grafted-chitosan (SA-g-CS) conjugate was synthesized by grafting SA onto CS via a free radical mediated method. Nanoparticles were prepared using casein (CA) together with SA-g-CS to improve the performance and sustained release of black rice anthocyanins (BRA). The results of UV-Vis, FTIR and H NMR spectra for SA-g-CS conjugates demonstrated the successful grafting of SA onto CS. The results of DPPH, ABTS and ferric ion reducing antioxidant power assays showed that the SA-g-CS conjugates had strong antioxidant capacities, and the higher the pH of the grafting reaction system, the stronger the antioxidant capacity of the conjugates. X-ray diffraction and scanning electron microscopy analyses showed that the crystallographic property and microstructure of CS were improved by the grafting of SA. Compared with BRA loaded nanoparticles prepared with CA alone or the combination of CS and CA, the BRA loaded nanoparticles constructed by SA-g-CS and CA have smaller particle size, better dispersion, encapsulation efficiency and sustained-release property. These results provided great potential for the application of phenolic acid grafted CS in stabilizing ACNs.
花色苷（ACNs）因其优异的健康益处而引起了相当多的研究关注，但由于其低稳定性和生物利用度，限制了它们的应用。在本研究中，通过自由基介导的方法将芥子酸接枝到壳聚糖（CS）上合成了芥子酸接枝壳聚糖（SA-g-CS）缀合物。使用酪蛋白（CA）与 SA-g-CS 一起制备纳米粒子，以提高黑米花色苷（BRA）的性能和缓释效果。UV-Vis、FTIR 和 H NMR 光谱的结果表明 SA-g-CS 缀合物成功地将 SA 接枝到 CS 上。DPPH、ABTS 和铁离子还原抗氧化能力测定的结果表明，SA-g-CS 缀合物具有较强的抗氧化能力，且接枝反应体系的 pH 值越高，缀合物的抗氧化能力越强。X 射线衍射和扫描电子显微镜分析表明，SA 的接枝改善了 CS 的结晶性能和微观结构。与单独用 CA 制备的 BRA 负载纳米粒子或 CS 和 CA 的组合相比，由 SA-g-CS 和 CA 构建的 BRA 负载纳米粒子具有更小的粒径、更好的分散性、包封效率和缓释性能。这些结果为酚酸接枝 CS 在稳定 ACNs 方面的应用提供了巨大的潜力。
