Institute for Agro-Products Processing and Nuclear-Agricultural Technology, Hubei Academy of Agricultural Science, Wuhan 430064, China; School of Bioengineering and Food Science, Hubei University of Technology, Wuhan 430068, China.
Institute for Agro-Products Processing and Nuclear-Agricultural Technology, Hubei Academy of Agricultural Science, Wuhan 430064, China.
Food Res Int. 2023 Feb;164:112361. doi: 10.1016/j.foodres.2022.112361. Epub 2023 Jan 2.
This study investigated the transport stress (crowding stress and duration) on the physicochemical properties, energy metabolism and antioxidant enzyme activities of the red swamp crayfish (Procambarus clarkii) tail muscle (CTM). Besides, transcriptomic and metabolomic were conducted to elucidate the possible mechanism of CTM alternations during transport stress. The survival rate of crayfish gradually decreased with the external crowding stress and crowding time increasing. The transport stress also led to the increased distance among muscle fibers, water mobility and energy consumption, and the decreased of water holding capacity (WHC), hardness of CTM. The hepatopancreas exhibited more sensitive to crowding stress than muscle. The multi-omics analysis revealed that transport stress could interfere the translation and protein folding functions of ribosomal proteins, fatty acid metabolism and degradation, physiological functions of mitochondria in CTM. This study could provide critical information to increase the understanding of the regulation mechanism of crayfish when subjected to transport stress.
本研究调查了运输应激（拥挤应激和持续时间）对红沼泽螯虾（Procambarus clarkii）尾部肌肉（CTM）理化性质、能量代谢和抗氧化酶活性的影响。此外，还进行了转录组学和代谢组学分析，以阐明 CTM 在运输应激过程中发生变化的可能机制。随着外部拥挤应激和拥挤时间的增加，螯虾的存活率逐渐下降。运输应激还导致肌肉纤维之间的距离增加、水流动性和能量消耗增加，而水保持能力（WHC）、CTM 的硬度降低。肝胰腺对拥挤应激比肌肉更敏感。多组学分析表明，运输应激会干扰核糖体蛋白的翻译和蛋白质折叠功能、脂肪酸代谢和降解、CTM 中线粒体的生理功能。本研究可以为增加对运输应激下螯虾调控机制的理解提供关键信息。
