State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
Int J Mol Sci. 2024 Jan 11;25(2):943. doi: 10.3390/ijms25020943.
Red swamp crayfish, (), is an important model crustacean organism used in many types of research. However, the effects of different doses of aminomethylphosphonic acid (AMAP) on the transcriptome and metabolites of have not been explored. Thus, this study investigated the molecular and metabolic mechanisms activated at the different exposure dosages of AMAP in to provide new insights into the strategies of in response to the high concentrations of AMAP in the environment. In the present study, the were divided into three groups (control group; low-dosage AMAP exposure; high-dosage AMAP exposure), and hepatopancreatic tissue samples were dependently taken from the three groups. The response mechanisms at the different dosages of AMAP were investigated based on the transcriptome and metabolome data of . Differentially expressed genes and differentially abundant metabolites were identified in the distinct AMAP dosage exposure groups. The genes related to ribosome cell components were significantly up-regulated, suggesting that ribosomes play an essential role in responding to AMAP stress. The metabolite taurine, involved in the taurine and hypotaurine metabolism pathway, was significantly down-regulated. may provide feedback to counteract different dosages of AMAP via the upregulation of ribosome-related genes and multiple metabolic pathways. These key genes and metabolites play an important role in the response to AMAP stress to better prepare for survival in high AMAP concentrations.
罗氏沼虾，（），是一种重要的模式甲壳类生物，用于许多类型的研究。然而，不同剂量的氨甲基膦酸（AMAP）对罗氏沼虾转录组和代谢物的影响尚未得到探索。因此，本研究探讨了 AMAP 在不同暴露剂量下对 中激活的分子和代谢机制，为 应对环境中高浓度 AMAP 的策略提供新的见解。在本研究中，将 分为三组（对照组；低剂量 AMAP 暴露；高剂量 AMAP 暴露），并分别从三组中取肝胰腺组织样本。基于 的转录组和代谢组数据，研究了不同 AMAP 剂量的反应机制。在不同的 AMAP 剂量暴露组中鉴定出差异表达的基因和差异丰度的代谢物。与核糖体细胞成分相关的基因显著上调，表明核糖体在应对 AMAP 应激中起重要作用。涉及牛磺酸和次牛磺酸代谢途径的代谢物牛磺酸显著下调。可能通过上调核糖体相关基因和多种代谢途径来提供反馈以对抗不同剂量的 AMAP。这些关键基因和代谢物在应对 AMAP 应激中发挥重要作用，以更好地为在高 AMAP 浓度下生存做好准备。
