Abacı Nurten, Senol Deniz F Sezer, Ekhteiari Salmas Ramin, Uysal Bayar Fatma, Turgut Kenan, Orhan Ilkay Erdogan
Department of Pharmacognosy, Gazi University, Faculty of Pharmacy, Ankara, Türkiye.
Department of Chemistry, King's College London, Britannia House, London, UK.
Int J Environ Health Res. 2024 Feb;34(2):674-686. doi: 10.1080/09603123.2022.2163988. Epub 2023 Feb 5.
The EtOH extracts of the leaves of two new cultivars (Uysal-SFU and Turgut-SFT) of Salvia fruticosa Mill. was tested against acetylcholinesterase (IC50: 30.62 ± 3.27 and 32.97 ± 2.33 µg/mL for SFU and SFT, respectively) and butyrylcholinesterase (IC50: 69.91 ± 1.08 µg/mL and 86.55 ± 1.26 µg/mL), respectively, relevant to Alzheimer's disease. The essential oils showed a stumpy inhibition against AChE and no inhibition against BChE. DPPH radical scavenging activity of the extracts (86.70 ± 0.17% and 86.14 ± 1.13% for SFU and SFT, respectively) was stronger than that of quercetin (85.51 ± 0.17%): Their (1.24 ± 0.05 and 1.04 ± 0.16 for SFU and SFT, respectively) ferric-reducing antioxidant power were close to that of the reference (e.g. quercetin, 1.42 ± 0.14). Molecular docking simulations were performed on their major monoterpenes. Our findings revealed that the leaf EtOH extracts of two cultivars are promising inhibitors of both AChE and BChE.
对两种新培育的药用鼠尾草（Uysal-SFU和Turgut-SFT）叶片的乙醇提取物针对与阿尔茨海默病相关的乙酰胆碱酯酶（SFU和SFT的IC50分别为30.62±3.27和32.97±2.33μg/mL）和丁酰胆碱酯酶（IC50分别为69.91±1.08μg/mL和86.55±1.26μg/mL）进行了测试。精油对乙酰胆碱酯酶显示出微弱的抑制作用，对丁酰胆碱酯酶无抑制作用。提取物的DPPH自由基清除活性（SFU和SFT分别为86.70±0.17%和86.14±1.13%）强于槲皮素（85.51±0.17%）：它们的铁还原抗氧化能力（SFU和SFT分别为1.24±0.05和1.04±0.16）接近参考物（如槲皮素，1.42±0.14）。对其主要单萜进行了分子对接模拟。我们的研究结果表明，两个品种的叶片乙醇提取物有望成为乙酰胆碱酯酶和丁酰胆碱酯酶的抑制剂。
