Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey.
Food Chem Toxicol. 2013 Sep;59:96-103. doi: 10.1016/j.fct.2013.05.053. Epub 2013 Jun 10.
Inhibitory effect of the n-hexane, dichloromethane, ethyl acetate, and ethanol extracts from Anethum graveolens L. (dill) cultivated under organic (AG-O) and conventional (AG-C) conditions was tested against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and tyrosinase at 200 μg mL⁻¹. Their antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), N,N-dimethyl-p-phenylendiamine (DMPD), and nitric oxide (NO) radical scavenging assays as well as ferric ion-chelation capacity, ferric-(FRAP), and phosphomolybdenum-reducing antioxidant power (PRAP). The phytochemical analyses have been performed on both of the plant samples. GC-MS analysis pointed out that α-phellandrene was the main component in both of the essential oils in varying amounts (47.75% for AG-O and 27.94% for AG-C), while oleic acid was the dominant in the fruit oils of two samples (36.39% for AG-O and 53.87% for AG-C). HPLC analysis showed that both of the extracts contained rosmarinic acid as the major phenolic acid. The extracts inhibited BChE at moderate level, while the ethanol extracts exerted remarkable NO scavenging effect. The results emphasize that cultivation conditions may have effect on bioactivity and phytochemical content on plant samples.
在 200μg/mL 浓度下，分别测试了有机（AG-O）和常规（AG-C）种植条件下的莳萝（Anethum graveolens L.）的正己烷、二氯甲烷、乙酸乙酯和乙醇提取物对乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BChE）和酪氨酸酶的抑制作用。使用 2,2-二苯基-1-苦基肼基（DPPH）、N,N-二甲基-对苯二胺（DMPD）和一氧化氮（NO）自由基清除测定法以及铁离子螯合能力、铁还原抗氧化能力（FRAP）和磷钼酸盐还原抗氧化能力（PRAP）来测定其抗氧化活性。对两种植物样品进行了植物化学分析。GC-MS 分析表明，α-蒎烯是两种精油中的主要成分，其含量不同（AG-O 为 47.75%，AG-C 为 27.94%），而油酸是两种样品果实油中的主要成分（AG-O 为 36.39%，AG-C 为 53.87%）。HPLC 分析表明，两种提取物均含有迷迭香酸作为主要的酚酸。提取物对 BChE 有中等抑制作用，而乙醇提取物对 NO 有显著的清除作用。结果强调了栽培条件可能对植物样品的生物活性和植物化学物质含量有影响。
