Mannacharaju Mahesh, Ganesan Sekaran, Lee Jung-Kul, Rajagopal Rajinikanth, Chang Soon Woong, Ravindran Balasubramani
Environmental Science and Engineering Division, CSIR - Central Leather Research Institute (CLRI), Adyar, Chennai, 20, TN, India; Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul, 05029, Republic of Korea.
Environmental Science and Engineering Division, CSIR - Central Leather Research Institute (CLRI), Adyar, Chennai, 20, TN, India; SRM Institute of Science and Technology, Ramapuram Campus, Chennai-600089, India.
Chemosphere. 2023 Apr;320:138022. doi: 10.1016/j.chemosphere.2023.138022. Epub 2023 Feb 3.
In conventional, the biologically treated tannery wastewaters are rich in dissolved organics and the application of reverse osmosis (RO) to biologically treated tannery wastewater was challenged with fouling and failure of RO membrane due to existence of lingering dissolved organic compounds. In present investigation the bacterial cell immobilized packed bed reactor (CIPBR) was operated to remove the dissolved organic compounds in biologically treated post-tanning wastewater to avoid membrane fouling in RO. The efficient microbial syndicate to eliminate dissolved organics in post-tanning wastewater was isolated and immobilized on to the carbon silica matrix (CSM) in the range of 2.98 ± 0.2 × 10 cells gm of CSM and the same was used as a carrier matrix in the packed bed reactor. The CIPBR established the COD, COD and BOD removal efficiency by 61 ± 4%, 57 ± 4% and 87 ± 3% respectively with COD, COD and BOD remained in the treated wastewater as 236 ± 21 mg/L, 228 ± 21 mg/L, and 12 ± 3 mg/L under continuous operation. The removal of dissolved organic compounds from the post-tanning wastewater was confirmed using UV-Visible and FT-IR spectroscopic studies. Among the total microbial community, the phylum Proteobacteria played most abundant role with 48.47% of relative abundance for the removal of dissolved organics in biologically treated post-tanning wastewater. The significance of the study is to replace the tertiary treatment unit operation in the conventional ETP/CETP to remove dissolved organics in wastewater.
传统上，经过生物处理的制革废水富含溶解性有机物，将反渗透（RO）应用于经过生物处理的制革废水时，由于残留的溶解性有机化合物的存在，RO膜面临着污染和失效的挑战。在本研究中，运行细菌细胞固定化填充床反应器（CIPBR）以去除经过生物处理的鞣后废水中的溶解性有机化合物，以避免RO中的膜污染。分离出用于消除鞣后废水中溶解性有机物的高效微生物群落，并将其固定在碳硅基质（CSM）上，固定量为每克CSM 2.98±0.2×10个细胞，该碳硅基质用作填充床反应器中的载体基质。CIPBR在连续运行时，对化学需氧量（COD）、化学需氧量和生化需氧量（BOD）的去除效率分别为61±4%、57±4%和87±3%，处理后废水中的COD、化学需氧量和BOD分别为236±21mg/L、228±21mg/L和12±3mg/L。通过紫外可见光谱和傅里叶变换红外光谱研究证实了鞣后废水中溶解性有机化合物的去除。在整个微生物群落中，变形菌门在去除经过生物处理的鞣后废水中的溶解性有机物方面发挥了最主要的作用，相对丰度为48.47%。该研究的意义在于取代传统污水处理厂/集中污水处理厂中的三级处理单元操作，以去除废水中的溶解性有机物。
