State Key Laboratory of Urban Water Resource and Environment, School of Environmental, Harbin Institute of Technology, Harbin, 150090, China.
Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China.
Chemosphere. 2023 Apr;321:137983. doi: 10.1016/j.chemosphere.2023.137983. Epub 2023 Feb 4.
Microbes play a dominant role for the transformation of organic contaminants in the environment, while a significant gap exists in understanding the degradation mechanism and the function of different species. Herein, the possible bio-degradation of triclosan in microbial fuel cell was explored, with the investigation of degradation kinetics, microbial community, and possible degradation products. 5 mg/L of triclosan could be degraded within 3 days, and an intermediate degradation product (2,4-dichlorophen) could be further degraded in system. 32 kinds of dominant bacteria (relative intensity >0.5%) were identified in the biofilm, and 10 possible degradation products were identified. By analyzing the possible involved bioreactions (including decarboxylation, dehalogenation, dioxygenation, hydrolysis, hydroxylation, and ring-cleavage) of the dominant bacteria and possible degradation pathway of triclosan based on the identified products, biodegradation mechanism and function of the bacteria involved in the degradation of triclosan was clarified simultaneously. This study provides useful information for further interpreting the degradation mechanism of organic pollutants in mixed flora by combining analysis microbiome community and degradation pathway.
微生物在环境中有机污染物的转化中发挥着主导作用，但对于不同物种的降解机制和功能，我们仍存在很大的认识空白。在此，我们探索了三氯生在微生物燃料电池中的可能生物降解途径，研究了降解动力学、微生物群落和可能的降解产物。在 3 天内可以降解 5mg/L 的三氯生，并且在系统中可以进一步降解中间降解产物（2,4-二氯苯酚）。在生物膜中鉴定出 32 种优势细菌（相对强度>0.5%），并鉴定出 10 种可能的降解产物。通过分析优势细菌可能涉及的生物反应（包括脱羧、脱卤、双氧、水解、羟化和环裂解）以及基于鉴定产物的三氯生可能的降解途径，同时阐明了参与三氯生降解的细菌的生物降解机制和功能。本研究通过结合微生物群落分析和降解途径，为进一步解释混合菌群中有机污染物的降解机制提供了有用信息。
