Ahirwar Ankesh, Khan Mohd Jahir, Khandelwal Priyanka, Singh Gurpreet, Vinayak Vandana, Ghangrekar Makarand Madhao
Diatom Nano Engineering and Metabolism Laboratory (DNM), School of Applied Sciences, Dr. Harisingh Gour Central University, Sagar, Madhya Pradesh, 470003, India.
Metabolism, Bioengineering of Microalgal Metabolism and Applications (MIMMA), Research unit Biologie des Organismes Stress Santé Environnement, Le Mans University, IUML-FR 3473 CNRS, Le Mans, France.
Sci Rep. 2025 Aug 18;15(1):30196. doi: 10.1038/s41598-025-13271-1.
Power generation and recovery of value-added products using microalgae, Haematococcus lacustris is tested in a dual chamber photosynthetic microalgae-assisted microbial fuel cell (PMA-MFCt). The microalgal cells in conical flask act as control. The performance was compared to another, test PMA-MFCt. The control MFC in second test had electrode wires not connected (PMA-MFC). The PMA-MFCt set had microalgal catholytic media replenished unlike in PMA-MFCt. A comparative PMA-MFC, was used without microalgae and only water as catholyte. The results demonstrated maximum power density (PDmax) of 33.76 mW m in PMA-MFCt, 15.36 mW m in PMA-MFCt and 8.05 mW m in PMA-MFC. The non replenishment of catholytic media in PMA-MFCt set resulted in nutrient limitations, poor photosynthesis, and disrupted redox reactions. Further lowest PDmax in PMA-MFC proves that microalgae are excellent source of free nascent oxygen required for redox reaction. Taxonomic identity of microbes at the anode via 16 S rRNA showed the dominance of catalytic microbes mainly Proteobacteria. The different kinds of carotenoids from microalgae were estimated by UV-Vis and liquid chromatography-mass spectrometry (LC-MS) analysis. The microalgal growth, evaluated in terms of biomass dry weight (DW), was 118 mg L, after 40 days of PMA-MFCt operation, which was lesser than in control (conical flask) 123 mg L. The pigments including total chlorophyll (a + b), and total carotenoids were 699.7 µg g and 224.6 µg g, respectively, on day 16. Microalgal performance in PMA-MFCt and its control (PMA-MFC) was 10% and 32.52% inferior than in PMA-MFCt and its control. The continuous replenishment of media in PMA-MFCt maintained microalgal cells in continuous state of multiplication and photosynthesis resulting into higher bioelectricity generation and bioproducts than PMA-MFCt, and PMA-MFC.
利用微藻——湖生血球藻进行发电及增值产品回收，在双室光合微藻辅助微生物燃料电池（PMA-MFCt）中进行了测试。锥形瓶中的微藻细胞作为对照。将该性能与另一个测试PMA-MFCt进行比较。第二次测试中的对照MFC电极线未连接（PMA-MFC）。PMA-MFCt装置与PMA-MFCt不同，其微藻阴极电解液进行了补充。使用了一种无微藻且仅用水作为阴极电解液的对比PMA-MFC。结果表明，PMA-MFCt的最大功率密度（PDmax）为33.76 mW/m²，PMA-MFCt为15.36 mW/m²，PMA-MFC为8.05 mW/m²。PMA-MFCt装置中阴极电解液未补充导致营养限制、光合作用不佳以及氧化还原反应中断。PMA-MFC中最低的PDmax进一步证明微藻是氧化还原反应所需游离新生氧的极佳来源。通过16S rRNA对阳极微生物的分类鉴定表明，主要是变形菌门的催化微生物占主导。通过紫外-可见光谱和液相色谱-质谱（LC-MS）分析对微藻中的不同类胡萝卜素进行了估算。在PMA-MFCt运行40天后，以生物量干重（DW）衡量的微藻生长量为118 mg/L，低于对照（锥形瓶）的123 mg/L。在第16天，包括总叶绿素（a + b）和总类胡萝卜素在内的色素分别为699.7 μg/g和224.6 μg/g。PMA-MFCt及其对照（PMA-MFC）中的微藻性能分别比PMA-MFCt及其对照低10%和32.52%。PMA-MFCt中持续补充培养基使微藻细胞保持持续增殖和光合作用状态，从而产生比PMA-MFCt和PMA-MFC更高的生物电和生物产品。
