State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong 271018, PR China.
Pestic Biochem Physiol. 2023 Feb;190:105333. doi: 10.1016/j.pestbp.2022.105333. Epub 2022 Dec 23.
Apis cerana cerana is a native bee species in China and plays a key role in agricultural production and ecological balance. However, the growth and development of Apis cerana cerana has not been smooth, and pesticide and heavy metal stress are key factors that have forced a dramatic decline in population size. This study was performed with the objective of investigating the role of AccCDK20 and AccCDKN1 in honey bee resistance to pesticide and heavy metal stress. RT-qPCR analysis revealed that AccCDK20 transcript levels were highest in brown-eyed pupae and AccCDKN1 transcript levels were highest in 1-day-old worker bees. In different tissues and body parts of adult bees, AccCDK20 transcript levels were highest in the head, and AccCDKN1 transcript levels were highest in the thorax. It was further observed that environmental stress can affect the transcript levels of the AccCDK20 and AccCDKN1 genes. Silencing of the AccCDK20 and AccCDKN1 genes resulted in altered activities of antioxidant-related genes and antioxidant-related enzymes. AccCDK20 and AccCDKN1 transcript levels were upregulated under glyphosate stress, and silencing of the genes resulted in reduced resistance to glyphosate and greatly increased mortality in Apis cerana cerana. In addition, gene function was verified by in vitro repression assays. Overexpression of the AccCDK20 and AccCDKN1 proteins in E. coli cells increased the resistance to ROS damage induced by CHP. In conclusion, AccCDK20 and AccCDKN1 play an indispensable role in honey bee resistance to pesticide and heavy metal stress.
中华蜜蜂是中国本土的一种蜜蜂物种，在中国农业生产和生态平衡中发挥着关键作用。然而，中华蜜蜂的生长发育并不顺利，农药和重金属胁迫是导致其种群数量急剧下降的关键因素。本研究旨在探讨 AccCDK20 和 AccCDKN1 在蜜蜂抵抗农药和重金属胁迫中的作用。RT-qPCR 分析显示，AccCDK20 的转录水平在棕眼蛹中最高，AccCDKN1 的转录水平在 1 日龄工蜂中最高。在成年蜜蜂的不同组织和身体部位中，AccCDK20 的转录水平在头部最高，AccCDKN1 的转录水平在胸部最高。进一步观察到，环境胁迫会影响 AccCDK20 和 AccCDKN1 基因的转录水平。AccCDK20 和 AccCDKN1 基因的沉默会改变抗氧化相关基因和抗氧化相关酶的活性。草甘膦胁迫下 AccCDK20 和 AccCDKN1 的转录水平上调，基因沉默会降低对草甘膦的抗性，使中华蜜蜂的死亡率大大增加。此外，通过体外抑制试验验证了基因功能。AccCDK20 和 AccCDKN1 蛋白在大肠杆菌细胞中的过表达增加了 CHP 诱导的 ROS 损伤的抗性。综上所述，AccCDK20 和 AccCDKN1 在蜜蜂抵抗农药和重金属胁迫中起着不可或缺的作用。
