Biology Department, Basic and Industrial Microbiology Section, Faculty of Science, Ege University, Izmir, 35040, Türkiye.
Nutrition and Dietetics Department, Faculty of Health Sciences, Bingöl University, Bingöl, 12000, Türkiye.
Chem Biodivers. 2023 Mar;20(3):e202201182. doi: 10.1002/cbdv.202201182. Epub 2023 Feb 17.
Propolis is a natural resinous mixture produced by the excretions of honeybees. PCR amplification of the 16S rRNA gene region was achieved using DNA of pre-enriched propolis samples collected from Apis mellifera production hives (n=37) in Eastern Türkiye (Bingöl and its regions). Next-generation sequencing and metabarcoding techniques were used to identify bacterial communities in propolis samples. Firmicutes dominated the phylum structure, with Proteobacteria, Actinobacteria, Tenericutes, and Spirochaetes following. The top three bacterial families were Bacillaceae, Enterobacteriaceae, and Enterococcaceae. Bacillus (dominantly B. badius and B. thermolactis at the species level) was recognized at the genus level, followed by Enterococcus and Clostridium sensu stricto. Our study comprehensively identified the bacterial diversity of propolis samples. Further investigations targeting to enlighten the microbiota of propolis and its potential application fields are required to gain better insight into ecological, nutritional, and medicinal perspectives.
蜂胶是一种由蜜蜂分泌物产生的天然树脂混合物。使用从土耳其东部（Bingöl 及其地区）的 Apis mellifera 生产蜂巢中收集的预富集蜂胶样本的 DNA，实现了 16S rRNA 基因区域的 PCR 扩增。下一代测序和代谢条形码技术用于鉴定蜂胶样本中的细菌群落。厚壁菌门在门结构中占主导地位，随后是变形菌门、放线菌门、无壁菌门和螺旋体门。排名前三的细菌科是芽孢杆菌科、肠杆菌科和肠球菌科。在属水平上识别出芽孢杆菌（主要是 B. badius 和 B. thermolactis 种水平），其次是肠球菌和严格梭菌。我们的研究全面鉴定了蜂胶样本的细菌多样性。需要进一步的研究来阐明蜂胶的微生物组及其潜在的应用领域，以从生态、营养和医学角度获得更深入的了解。
