Cao Changhong, Zhong Wenting, Gao Feng, Zhang Ying, Ma Zhiguo, Cao Hui, Wu Menghua
Lingnan Traditional Chinese Medicine Research Centre, School of Pharmaceutical Sciences, Jinan University, Guangzhou, Guangdong, China.
Lingnan Resource Sub-center, National Engineering Research Center for Modernization of Traditional Chinese Medicine, Guangzhou, Guangdong, China.
PLoS One. 2025 Apr 7;20(4):e0320731. doi: 10.1371/journal.pone.0320731. eCollection 2025.
The genetic analysis, particularly focusing on the psbA-trnH region, aims to tackle the challenges linked to myrrh identification and improve quality control in medicinal and aromatic plant sectors. This process reveals the genetic diversity inherent in myrrh species, identifies adulterants, and assesses consistency with pharmacopoeia-designated species.
A meticulous investigation was conducted, involving twenty-five myrrh samples sourced from diverse origins and one adulterant sample. The methodology encompassed precise execution of DNA extraction, PCR amplification targeting the psbA-trnH region, sequencing, and subsequent data analysis. Additionally, the integration of GenBank data was employed to enrich the genetic analysis.
The psbA-trnH region demonstrated 100% amplification efficiency across all myrrh samples, accurately identifying three distinct species-Commiphora gileadensis, Commiphora myrrha, and Commiphora edulis. Only 8% of samples aligned with pharmacopoeia-specified species, revealing a significant misalignment. The identified adulterant, Liquidambar formosana, underscored the efficacy of the genetic approach. Genetic distances and haplotype analysis offered insights into myrrh species diversity. Intraspecific and interspecific distances highlighted the discriminatory potential of the psbA-trnH region. A phylogenetic tree illustrated distinct genetic clusters among Commiphora species and Liquidambar formosana.
It affirms the robustness of the psbA-trnH region for authenticating myrrh and emphasizes the necessity of adapting pharmacopoeial standards to accurately mirror genetic diversity. An avenue for exploring therapeutic variations within myrrh species and advocates collaboration among researchers, regulatory agencies, and industry stakeholders to fortify comprehensive quality management measures within the context of agronomy-focused herbal products.
基因分析，特别是聚焦于psbA-trnH区域，旨在应对与没药鉴定相关的挑战，并改善药用和芳香植物领域的质量控制。这一过程揭示了没药物种固有的遗传多样性，识别掺假物，并评估与药典指定物种的一致性。
进行了一项细致的调查，涉及来自不同产地的25个没药样本和1个掺假物样本。该方法包括精确执行DNA提取、针对psbA-trnH区域的PCR扩增、测序以及后续的数据分析。此外，整合GenBank数据以丰富基因分析。
psbA-trnH区域在所有没药样本中显示出100%的扩增效率，准确识别出三种不同的物种——乳香没药树、没药树和可食没药树。只有8%的样本与药典规定的物种相符，显示出明显的不一致。鉴定出的掺假物枫香树强调了基因方法的有效性。遗传距离和单倍型分析提供了有关没药物种多样性的见解。种内和种间距离突出了psbA-trnH区域的鉴别潜力。系统发育树展示了没药属物种和枫香树之间不同的基因簇。
它肯定了psbA-trnH区域用于鉴定没药的稳健性，并强调了调整药典标准以准确反映遗传多样性的必要性。为探索没药物种内的治疗差异开辟了一条途径，并倡导研究人员、监管机构和行业利益相关者之间的合作，以加强以农艺为重点的草药产品背景下的全面质量管理措施。
