Hwang Wonjae, Choi Joong Kyung, Bang Moon Suk, Park Woong-Yang, Oh Byung-Mo
Department of Rehabilitation Medicine, Seoul National University College of Medicine, Seoul, Korea.
Department of Rehabilitation Medicine, Seoul National University Hospital, Seoul, Korea.
Brain Neurorehabil. 2022 Sep 30;15(3):e27. doi: 10.12786/bn.2022.15.e27. eCollection 2022 Nov.
Repetitive transcranial magnetic stimulation (rTMS) is gaining popularity as a research tool in neuroscience; however, little is known about its molecular mechanisms of action. The present study aimed to investigate the rTMS-induced transcriptomic changes; we performed microarray messenger RNA, micro RNA, and integrated analyses to explore these molecular events. Eight adult male Sprague-Dawley rats were subjected to a single session of unilateral rTMS at 1 Hz (n = 4) or sham (n = 4). The left hemisphere was stimulated for 20 minutes. To evaluate the cumulative effect of rTMS, eight additional rats were assigned to the 1-Hz (n = 4) or sham (n = 4) rTMS groups. The left hemisphere was stimulated for 5 consecutive days using the same protocol. Microarray analysis revealed differentially expressed genes in the rat cortex after rTMS treatment. The overrepresented gene ontology categories included the positive regulation of axon extension, axonogenesis, intracellular transport, and synaptic plasticity after repeated sessions of rTMS. A single session of rTMS primarily induced changes in the early genes, and several miRNAs were significantly related to the mRNAs. Future studies are required to validate the functional significance of selected genes and refine the therapeutic use of rTMS.
重复经颅磁刺激（rTMS）作为神经科学领域的一种研究工具正日益受到关注；然而，其分子作用机制却鲜为人知。本研究旨在探究rTMS诱导的转录组变化；我们进行了微阵列信使核糖核酸、微小核糖核酸及综合分析以探索这些分子事件。八只成年雄性斯普拉格-道利大鼠接受了单次1赫兹的单侧rTMS（n = 4）或假刺激（n = 4）。对左侧半球刺激20分钟。为评估rTMS的累积效应，另外八只大鼠被分配到1赫兹（n = 4）或假刺激（n = 4）rTMS组。按照相同方案对左侧半球连续刺激5天。微阵列分析揭示了rTMS治疗后大鼠皮质中差异表达的基因。在多次rTMS刺激后，过度表达的基因本体类别包括轴突延伸的正向调节、轴突发生、细胞内运输及突触可塑性。单次rTMS主要诱导早期基因的变化，并且一些微小核糖核酸与信使核糖核酸显著相关。未来研究需要验证所选基因的功能意义并优化rTMS的治疗应用。
